The FastBreak Cell Lysis Reagent, 10X is designed for efficient, gentle lysis of E. coli cultures without centrifugation or mechanical cell disruption. The reagent is added directly to E. coli cultures and following a brief incubation, the cells are disrupted and the protein of interest is released. Recombinant proteins can be directly screened in the cell extract, or purified by the addition of the appropriate affinity matrix. This format allows for manual or robotic purification.
Figure 1.
Purification of 6X polyhistidine-tagged fusion protein using FastBreak Cell Lysis Reagent, 10X and MagneHis™ Protein Purification System. Lane 1, bacterial cell lysate expressing 6X polyhistidine-tagged firefly luciferase lysed by the addition of FastBreak Cell Lysis Reagent; Lane 2, cell supernatant post lysis; Lane 3, flowthrough of cell lysate after the addition of MagneHis particles; Lane 4, eluted 6X polyhistidine-tagged firefly luciferase; Lane 5, protein marker.
Figure 2.
Reproducibility of automated procedure. Lanes denote the well number of a 96-well plate from which the sample cultures were lysed using the FastBreak Cell Lysis Reagent, 10X and purified using the MagneHis Protein Purification System; Lane M, protein marker.
FastBreak Cell Lysis Reagent, 10X - online catalog information
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