PCR Cloning

T-Vector Cloning: The easy way to clone PCR products

The pGEM® T and pTARGET™ T-Vector Systems make it easy to clone and express PCR products. Use the pGEM-T or pGEM-T Easy Vectors for general PCR cloning, and the pTARGET vector when you need to express the insert in mammalian cells.

Quick tips for PCR cloning (pdf)

Featured T-Vectors

General PCR Cloning: pGEM-T Vector Systems

  • Rapid and reliable
  • No need for post-PCR modification
  • Cloning is complete in one hour at room temperature
  • Better value than other systems
  • Easy release of the insert from the vector
View catalog listing See a quick protocol

Watch the video below to see how easy it is!

Direct Mammalian Expression: pTARGET™ Vector

  • Easily express cloned PCR products in mammalian cells
  • Powerful CMV promoter
  • Increased expression due to the SV40 late poly(A) sequence and  origin of replication
View catalog listing See a protocol