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The Wizard® SV 96 Genomic DNA Purification System
provides a fast, simple technique for the preparation of genomic DNA
from cultured cells and tissue, including mouse tails. Genomic DNA can
be purified from cultured cells in about 30 minutes and isolation of
tissue or mouse tails in less than one hour, with an overnight digest
with Proteinase K. Amplifiable genomic DNA can be isolated from up to
5 × 106 cells per prep, from 20mg of tissue or from up to 1.2cm of a
mouse tail tip without a centrifugation clearing step prior to
purification.
With the Wizard® SV 96 Genomic DNA Purification System,
genomic DNA is purified from cell lysates using 96-well vacuum
filtration, eliminating the need for centrifugation. Purification
requires the use of the Vac-Man®
96 Vacuum Manifold or similar 96-well manifold. The technique also
provides the ability for automation on liquid handling workstations
such as the Beckman Biomek® 2000.
High-throughput genomic DNA purification is made easy because the SV
96 System uses a convenient vacuum manifold apparatus that does not
require disassembly during the genomic DNA binding and wash steps.
Filtrate waste products are delivered directly to a vacuum trap,
eliminating the need to empty waste collection vessels. Purified
genomic DNA is eluted into a 96-well deep-well plate.
Figure 1. Genomic DNA isolation and purification
using the Wizard® SV 96 DNA Purification System
FEATURES
Fast: Obtain genomic DNA from 96 mouse tails in 45-60 minutes, cultured
cells in 30 minutes after lysis. No spin required.
Flexible: Purify genomic DNA from up to 20mg of tissue, 1.2cm of mouse tail or 5
x 106 cultured cells per well. Tissue isolations require Proteinase K.
Figure 2. Cross-contamination assay. Genomic
DNA purified from mouse tail clipping samples or water samples arrayed
in adjacent wells of 96-well plate. PCR products were amplified from 1µl
of purified sample from each well using primers for mouse IL-1ß. Ten
microliters of PCR product were separated on a 1% gel and visualized
by ethidium bromide staining. Expected IL-1ß PCR product from mouse
tail clippings is approximately 1.2kb. No PCR product was expected
from water samples. Reactions were amplified for 30 cycles.
High Yield: Purify 20-30µg of DNA per prep from 1.2cm mouse tail clips.
Figure 3. Purity (Panel A) and yield (Panel B) measurements from 96
separate 20mg mouse tail clippings using the Wizard® SV 96 Genomic
DNA Purification System.
Performance: Obtain purified DNA ready for direct amplification.
Automation: Sufficient reagents for either manual or automated purification
protocols.
Additional Information
DNA and RNA Purification
Automation-Robotics
Promega Notes
Vac-Man and Wizard is a trademark of Promega
Corporation and is registered with the U.S. Patent and Trademark
Office.
Biomek is a registered trademark of Beckman Instruments, Inc.
View Online Catalog Information
Wizard®
SV 96 Genomic DNA Purification System
Vac-Man®
96 Vacuum Manifold
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