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How Two-Step RT-PCR Works
Two-step RT-PCR protocols use either oligo(dT) primers or random primers to prime first-strand cDNA synthesis and reverse transcribe all messages
in a particular sample. Two-step protocols uncouple
reverse
transcription (RT) allowing you to perform multiple PCR and therefore answer many questions from a single RNA sample. Two-step
methods can be homogeneous by combining the RT and PCR steps. Gene-specific priming can be used as well.
We offer a diverse selection of reverse transcriptases including M-MLV, AMV and
ImProm-II™ Reverse Transcriptases for those who prefer to 'mix and match' their own
reaction components. Oligo(dT) Primers and Random Hexamer Primers for priming first-strand
synthesis are also available from Promega.
View Online Catalog Information
Reverse
Transcription System
ImProm-II™
Reverse Transcriptase
AMV Reverse
Transcriptase
M-MLV Reverse
Transcriptase
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