Amplification

Featured Applications

Hot Start PCR

Reduce mispriming and primer-dimer formation with hot start PCR, which provides higher specificity and allows room-temperature setup.

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Standard PCR

Experience improved PCR performance with GoTaq® DNA Polymerase products.

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Reverse Transcription PCR

Perform either one-step or two-step RT-PCR with a variety of kits and enzyme available.

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PCR Cloning

Subclone PCR and RT-PCR products rapidly and affordably using Promega T-Vectors, which are based on the pGEM®-5Zf(+) Vector backbone. Or a convenient system for cloning PCR products for expression in mammalian cells.

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qPCR

Perform real-time PCR using the Plexor™ System, an easy and cost-efficient multiplex-capable real-time PCR chemistry for highly specific and sensitive gene expression or SNP genotyping analysis.

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High-Fidelity PCR

Improve the fidelity of your amplifications by using a thermostable polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and possesses a 3´→5´ proofreading activity. Use high-fidelity PCR for applications such as mutagenesis or cloning.

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dNTPs

Promega is a primary manufacturer of dNTPs, offering bulk and custom options. Choose the dNTPs mixes or purchase individual tubes.

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