Sequencing, Mutagenesis & Labeling
DNA sequencing is often a fundamental requirement of molecular biology research.
We offer sequencing systems as well as products for upstream and downstream processing of your sequencing products.
Site-directed mutagenesis is a cornerstone technique in molecular biology, with utility in many fields of research, including basic studies of gene regulatory elements, DNA-protein interaction and protein structure/function. This page will help you find
the products and Technical Resources to assist in your research.
Sequencing Systems
SILVER SEQUENCE™ DNA Sequencing Systems
This non-radioactive system uses a silver-staining method for band detection, eliminating expensive instrumentation and reagents associated with other non-radioactive detection methods. High temperatures used with this system eliminate the need for alkaline denaturation of double-stranded DNA templates, prevent rapid reannealing of PCR products, increase the stringency of primer hybridization and reduce sequencing problems associated with secondary structure.
Learn more...Sequencing Primers
RNA Polymerase Promoter Sequencing Primers pUC/M13 Sequencing Primers Reporter Vector and Luciferase Sequencing Primers pTARGET™ Sequencing PrimersMutagenesis Systems
GeneEditor™ in vitro Site-Directed Mutagenesis System
This system uses antibiotic selection to obtain a high frequency of mutants. GeneEditor™ Selection Oligonucleotides provided with the GeneEditor™ System encode mutations that alter the ampicillin resistance gene, creating a new additional resistance to the GeneEditor™ Antibiotic Selection Mix. In the GeneEditor™ System protocol, the Selection Oligonucleotide is annealed to a single- or double-stranded DNA template at the same time as a mutagenic oligonucleotide. Subsequent synthesis and ligation of the mutant strand links the two oligonucleotides.
Learn more...Altered Sites® II in vitro Mutagenesis Systems
These systems provide the ability to mutagenize double-stranded template DNA, perform sequential rounds of mutagenesis without subcloning, and express the mutated gene products in vivo or in vitro. The Altered Sites® Systems use antibiotic selection as a means to obtain a high frequency of mutants.
Learn more...Altered Sites® II Mammalian in vitro Mutagenesis System
This system provides reagents to mutagenize double-stranded (ds) or single-stranded DNA (ssDNA) templates and to perform sequential rounds of mutagenesis without subcloning. Expression of sequences in mammalian cells is also possible without subcloning.
The system uses antibiotic selection to obtain a high frequency of mutants.
Nested Deletion Mutagenesis Systems
Erase-a-Base System
Designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA, this system is based on the procedure developed by Henikoff, in which exonuclease III (Exo III) is used to specifically digest insert DNA from a 5´ protruding or blunt end restriction site.
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