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Riboprobe® and RiboMAX™ Systems
Promega’s Riboprobe® in vitro Transcription Systems
are designed for in vitro preparation of high specific activity
single-stranded RNA probes or microgram quantities of defined RNA
transcripts from cloned DNA inserts.
Promega’s Riboprobe® Systems include the RNA
polymerases, all of the required reagents for performing
transcription reactions in vitro (excluding radioisotope) and RQ1
RNase-Free DNase for removal of the template following
transcription.
Promega also offers the RiboMAX™ Large Scale RNA Production
Systems, which produce milligram amounts of RNA.
Choose the System that's right for your
needs:
The RiboMAX™ Systems differ from the Riboprobe®
Systems in three primary ways:
1) A HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9)
buffer
2) rNTP and magnesium concentrations are elevated at levels
appropriate for either SP6, T7 or T3 RNA Polymerase (1)
3) Yeast inorganic pyrophosphatase is included in the reaction (2).
References
1. Gurevich, V.V. et al. (1991) Preparative in vitro mRNA synthesis
using SP6 and T7 RNA polymerases. Anal. Biochem. 195, 207.
2. Cunningham, P.R. and Ofengand, J. (1990) Use of organic
pyrophosphatase to improve the yield of in vitro transcription
reactions catalyzed by T7 RNA. Biotechniques 9, 713.
The proof is in the testing.
Promega manufactures all the technical components of these systems.
Our scientists test and calibrate the systems for optimal perfomance
and reliability in laboratory conditions. The results can be
reviewed by requesting part number BR102 from the online
document request form. You may also download the information here
(.pdf file - 409 kbytes)
Questions?
Our Technical Services scientists are available to answer questions
about our systems and how to apply them to your laboratory
environment. If you have questions, please e-mail techserv@promega.com
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