In Vitro Transcription & Microarrays
Many techniques have been developed to examine absolute and relative levels of gene expression. From Northern blots and RNase protection assays to microarrays and qPCR, Promega provides flexible options to meet your expression analysis needs.Featured Applications
Microarray Analysis
Generate consistent, reproducible and sensitive
microarray data with the ChipShot™ Systems. With ChipShot™ you can generate fluorescently labeled cDNA, which can then
be hybridized to a printed microarray.
qPCR & qRT-PCR
Plexor® Real-Time qPCR and qRT-PCR Systems Systems offer you:
- Multiplex Performance: Only two primers are required for each target, making design of multiplex assays easier.
- Improved Productivity: Less labor, less time and less cost per assay. Measure controls and targets at the same time in the same well.
- Convenience: The master mix format provides everything you need in one tube.
In Vitro Transcription
With the Riboprobe® in vitro Transcription Systems you can generate high-specific-activity, single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.
See Riboprobe® Systems Catalog Listing
The RiboMAX™ Large Scale RNA Production Systems consistently produce 2–5mg/ml of RNA in a 1ml reaction.
- Flexible: Systems for use with SP6 and T7 RNA polymerases.
- Scalable: Scale reactions up or down to suit your production requirements.
- High-Quality: Translation-grade RNA
RNA Analysis
The
RNA Analysis Notebook provides an introduction to RNA purification, RT-PCR, in vitro
transcription and
RNA analysis by microarray. You can download
the entire notebook in pdf format or select the chapter that best suits your research
interests.