This list will be updated as additional information becomes available. Contact
Promega Technical Services (genetic@promega.com)
for the latest information on available protocols.
|
Table 1. Sample Types Examined Using the Standard Protocol for the
DNA IQ™ System (see the DNA IQ™
System—Small Sample Casework Technical Bulletin
#TB296 or
DNA IQ™ System—Database Protocol Technical
Bulletin #TB297.)
|
| Sample Type |
Promega |
External |
Comments |
| Fresh blood |
Yes |
Yes |
Works with the following anti-clotting reagents:
EDTA, citrate, heparin, ACD.
See the DNA IQ™ System—Database
Protocol Technical Bulletin #TB297. |
|
Frozen blood |
Yes |
Yes |
Old blood may produce lower yields.
See the DNA IQ™ System—Database
Protocol Technical Bulletin #TB297. |
|
Blood stains on: |
|
|
See the DNA IQ™
System—Small Sample Casework Technical
Bulletin #TB296 and DNA IQ™
System—Database Protocol Technical Bulletin #TB297
to isolate DNA from stains on solid material. |
S&S903
paper |
Yes |
Yes |
|
FTA® paper |
Yes |
Yes |
| Cotton |
Yes |
Yes |
| Blue denim |
Yes |
Yes |
| Black denim |
Yes |
Yes |
| Soil |
Yes |
|
| Leather |
Yes |
Yes |
|
Buccal swabs* |
|
|
See the DNA IQ™ System—Database Protocol Technical
Bulletin #TB297 to isolate DNA from buccal swabs.
The expected DNA yield from a single buccal swab is up to 300ng. |
| Cotton |
Yes |
Yes |
| Rayon |
Yes |
|
| CEP paper |
Yes |
|
Swab to FTA®
paper |
|
Yes |
Foam swab to
paper |
|
Yes |
|
Cigarette butt |
Yes |
Yes |
Cut paper wrapping into small pieces. Add 200μl of Incubation Buffer from the Tissue
and Hair Extraction Kit (for use with DNA IQ™).
Incubate at 56°C for 30 minutes. Transfer buffer and sample to a
DNA IQ™ Spin Basket (Cat.# V1221). Centrifuge at room temperature
for 2 minutes at maximum speed. Discard Spin Basket. Add 400μl of prepared Lysis
Buffer from the DNA IQ™ System, and proceed to Step 5 of Section
4.B of the DNA IQ™
System—Small Sample Casework Technical Bulletin
#TB296.
The filter may form a gel if heated. |
|
Toothbrush |
|
Yes |
Preincubation in Lysis Buffer is recommended. |
|
Differential extractions |
|
Yes |
See the DNA IQ™ System—Small Sample Casework Technical
Bulletin #TB296. |
|
Semen stain (vasectomized) |
|
Yes |
Swabs containing skin epithelial cells were incubated with 250μl of prepared Lysis
Buffer for 30 minutes at 95°C. |
| Envelope |
|
Yes |
Soak in 0.5% SDS before adding 2 volumes
of prepared Lysis Buffer. |
|
Urine |
|
Yes |
Pellet cells, discard liquid and
add 100μl of prepared Lysis Buffer and 7μl of DNA IQ™
Resin to the cell pellet. Alternatively, add 2 volumes of prepared Lysis Buffer
and 7μl of DNA IQ™ Resin to liquid urine.
Proceed with Step 4 of Section 4.C of the DNA IQ™
System—Small Sample Casework Technical Bulletin
#TB296. |
|
Chewing gum |
|
Yes |
Mince gum into small pieces.
Preincubation in Lysis Buffer is recommended. |
|
Skin epithelial cells |
|
Yes |
Swabs of skin epithelial cells were incubated in 250μl of
Lysis Buffer at 95°C for 30 minutes. |
|
Saliva |
|
Yes |
|
|
Vomit |
|
Yes |
Preincubation in Lysis Buffer is recommended. |
|
Toothpick |
|
Yes |
Preincubation in Lysis Buffer is recommended. |
|
Knit cap |
|
Yes |
Preincubation in Lysis Buffer is recommended. |
|
Glove lining |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Cotton with hand soap |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Cotton with motor oil |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Cotton with hand cream |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Cotton with contraceptive foam |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Cotton with dirt |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Canvas |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Carpet |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
| Black underwear |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Plastic lining of a sanitary bag |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Pantyhose |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Blood-stained car visor |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
|
Bloodstains from tape |
|
Yes |
Preincubation
in Lysis Buffer is recommended. |
*Swabs also have been used to collect DNA from a variety of samples
and surfaces, including drinking glasses, aluminum cans, phone
handles, earrings, car door panels, steering wheels, fingernails and
jacket cuffs. Protocols for DNA isolation from these swabs would be
similar to the buccal swab protocol.
|
|
Samples Requiring Proteinase K Digestion
|
|
Table 2. Sample Types That Require Proteinase K
Treatment Prior to DNA Purification Using the DNA IQ™ System. |
DNA from the following sample types has been successfully purified at Promega or
by external forensic laboratories. Due to the nature of casework samples, (i.e.,
samples may have been exposed to environmental factors for long periods of time,
and the amount of biological material may be limiting) DNA yields may vary and
DNA may not be obtained from all samples.
The following sample types require a Proteinase K digestion prior to the
addition of two volumes of prepared Lysis Buffer to one volume of sample when following the
Tissue and Hair Extraction Kit (for use with DNA IQ™)
protocol. See Technical Bulletin #TB307. A separate protocol for bone and antler
samples is linked below.
|
| Sample Type |
Promega |
External |
Comments |
| Tissue |
|
|
|
| Fresh |
Yes |
Yes |
See the Tissue and Hair Extraction Kit (for use with DNA
IQ™) Technical Bulletin
#TB307. |
| Formalin fixed |
Yes |
Yes |
See the Tissue and Hair
Extraction Kit (for use with DNA IQ™)
Technical Bulletin #TB307. |
|
Hair roots |
Yes |
Yes |
See the Tissue and Hair
Extraction Kit (for use with DNA IQ™)
Technical Bulletin #TB307. |
|
Hair shafts (mitochondrial DNA) |
Yes |
|
See the Tissue and Hair Extraction Kit
(for use with DNA IQ™)
Technical Bulletin #TB307. |
|
Bone |
|
Yes |
From pulverized bone samples.
See the Bone protocol
for the DNA IQ™ System. |
|
Antler |
|
Yes |
From drill shavings.
See the Bone
Protocol for the DNA IQ™ System. |
|
Sweat-stained clothing |
|
Yes |
Soak cuttings in freshly prepared
Incubation Buffer/Proteinase K Solution for several hours. Add 2 volumes of
prepared Lysis Buffer and 7μl of DNA IQ™ Resin to the supernatant.
Proceed to Step 6 of Section 4.B of the DNA IQ™
System—Small Sample Casework Technical Bulletin
#TB296. |
|
Sperm |
Yes |
Yes |
A proteinase K digestion for 5–15 minutes is required
if the sperm is not from a differential extraction. Add 2 volumes of
prepared Lysis Buffer and 7μl of DNA IQ™ Resin. Proceed to Step 4 of
Section 2.C of the DNA IQ™ System—Small Sample
Casework Technical Bulletin #TB296. |
|
Fetal Tissue |
|
Yes |
|
