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Tissue and Hair Extraction Kit
Description
Promega’s DNA IQ™ System, which uses a novel paramagnetic resin to purify DNA,
contains a strong denaturing agent that disrupts many sample types in preparation
for DNA purification. However, some samples such as tissue are not sufficiently
disrupted with the DNA IQ™ Lysis Buffer and thus require pretreatment with Proteinase
K to ensure sample lysis. The Tissue and Hair Extraction Kit (for use with DNA IQ™)
includes Proteinase K and other reagents to break up most tissue samples and remove
proteins and other components from the DNA. The DNA can then be purified using the DNA IQ™ System.
The reagents and protocol supplied with the Tissue and Hair Extraction Kit (for use with DNA IQ™)
allow purification of DNA from a large variety of tissue types when combined with the DNA IQ™ System.
Both double-stranded or single-stranded genomic and mitochondrial DNA can be purified. The Proteinase
K digestion eliminates material that competes for binding sites on the DNA IQ™ Resin. As a result,
the resin will typically bind up to 3 times more DNA from Proteinase K-treated samples. The ability
to obtain DNA from samples depends on the quality of the starting materials. Tissue that has been
exposed to high temperatures for a considerable period of time may yield severely damaged DNA that
may not amplify well.
The DNA IQ™ System has been tested with the AluQuant® Human DNA Quantitation System and the
PowerPlex® 16 System to ensure a streamlined process. This translates into reliable products
giving optimal results from isolation to quantitation and STR analysis.
Features
- No feature list is available for this product.
System Components
- » Incubation Buffer
- » Proteinase K
- » DTT
- » Protocol
FAQ
» No FAQ is available for this product.
Related Information
- No related information is available for this product.
Figures
No figures are available for this product.
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