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Description
The PowerPlex® ES System is a multiplex STR system for use in DNA typing including
paternity testing, forensic DNA analysis, human identity testing, and tissue
culture strain identification. This system co-amplifies the loci D3S1358, TH01,
D21S11, D18S51 (all labeled with fluorescein), and Amelogenin, vWA, D8S1179, FGA
(all labeled with TMR), and SE33 (also known as ACTBP2, labeled with JOE).
The forensics community in Europe agreed upon 7 core STR as the European standards
for use in the convicted offender database (ENFSI recommended panel).
The PowerPlex® ES System includes these 7 core STR loci for ENFSI, as well
as Amelogenin and the highly polymorphic SE33 locus, which has more than
65 alleles and an exceptional matching probability of 1 in 114.94*. By
combining the 7 ENSFI loci and SE33, the PowerPlex® ES System provides
a matching probability of 1 in 5.914 × 1010 and a power of exclusion of 0.999946*
Allele determination is simplified by the inclusion of an allelic ladder for all
nine loci, which includes many frequently occurring microvariants. The amplified
STRs can be detected using the Applied Biosystems 3130 and 3130xl Genetic Analyzers, the ABI PRISM 3100 and 3100-Avant Genetic Analyzers, and the ABI PRISM 310 Genetic Analyzer.
* Population statistics data in American Caucasians from Budowle, B. et al.
(1999) J. Forensic Sci. 44, 1277–86.
Features
Amplify DNA Once, Not Twice:-
Simultaneous amplification of 9 DNA markers, including the 7 ENSFI loci, SE33, and Amelogenin.
Allelic Ladders:- Allelic Ladders for all 9 loci are provided to simplify allele determination and include
most frequently occurring microvariants.
Maximum Sensitivity:-
The PowerPlex® ES System provides maximum sensitivity—it is optimized
for use with 0.5–1ng of DNA.
Additional studies show interpretable and reproducible results can be obtained with <0.5ng of DNA.
Internal Lane Standard 600:-
This marker offers the greatest precision available for DNA typing. It is designed for use in each
gel lane or capillary to increase the precision of your analyses. The CXR-labeled marker consists
of 22 hands ranging in size from 60–600bp. Fragments that are multiples of 100 bases have fluorescence
intensities twice that of other fragments to simplify size assignment.
PowerTyper™ Macros:-
The PowerTyper™ Macros allows automatic assignment of genotypes using Genotyper® software.
PowerPlex® ES Separation Control:-
The Separation Control contains 5 SE33 allele and is used to document the resolution of
closely migrating fragments.
Instrumentation Compatibility:-
Amplification products are compatible with the Applied Biosystems 3130 and 3130xl Genetic Analyzers, the ABI PRISM 3100 and 3100-Avant Genetic Analyzers, and the ABI PRISM 310 Genetic Analyzer.
Outstanding Technical Support:- Promega Technical Services Representatives are highly trained scientists
who provide exceptional support for the entire Promega product line.
Promega also provides technical training and
support for its global distribution network and conducts customer
training workshops in human DNA typing.
System Components
- » PowerPlex® ES Primer Pair Mix
- » PowerPlex® ES Allelic Ladder Mix
- » PowerPlex® ES Separation Control
- » Gold ST*R 10X Buffer
- » 9947A DNA
- » Blue Dextran Loading Solution
- » Internal Lane Standard 600
FAQ
» No FAQ is available for this product.
Figures
- » No figures are available for this product.
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