|
Description
The PowerPlex® 16 BIO System is a multiplex STR system for use in DNA typing,
including paternity testing, forensic DNA analysis, human identity testing and
tissue culture strain identification. The System allows the coamplification and
three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The
PowerPlex® 16 BIO System co-amplifies the loci Penta E, D18S51, D21S11, TH01,
D3S1358 (labeled with fluorescein); FGA, TPOX, D8S1179, vWA, Amelogenin
(labeled with Rhodamine Red™-X); and Penta D, CSF1PO, D16S539, D7S820, D13S317,
D5S818 (labeled with JOE). The amplified STRs are detected using the Hitachi
FMBIO® II Fluorescence Imaging System.
High-throughput analysis is achieved by using the available software to compare
amplified sample DNA fragments directly with the allelic ladder present for each
locus. The allelic ladder mixture consists of most or all of the known alleles
in the population and is included to allow rapid and precise assignment of sample
alleles. The size marker (Internal Lane Standard 600 BIO) provided with the system
is tagged with a fourth fluorescent dye. This marker may be included as a standard
to increase precision in analysis when using the PowerPlex® 16 BIO System.
The Matrix 16 BIO is provided to assist in properly generating a color matrix for
color separation using a filter set* and the Hitachi FMBIO® Analysis Software. The
Matrix 16 BIO consists of fragments labeled with four different fluorescent dyes.
The four different fluorescent dyes are Fluorescein, JOE, Rhodamine Red™-X and
Texas Red®-X. These four dyes are the same ones in the PowerPlex®
16 BIO System.
*Filter sets (MiraiBio Cat.# 11999-246-00) are available for purchase from MiraiBio,
Inc., at 800-624-6176 (USA) or 510-337-2000.
Features
Amplify DNA Once, Not Twice:- No longer do you have to amplify each database or casework sample twice.
With the PowerPlex® 16 BIO System you can amplify all 13 CODIS STR loci at one time.
Maximum Sensitivity:- The PowerPlex® 16 BIO System is designed to work with 0.5ng of DNA template.
Allelic Ladders Mix:- Allelic ladders for all 16 loci, labeled with three fluorescent dyes,
are provided to assure balance across loci and simplify interpretation of alleles.
Internal Lane Standard (ILS) 600 BIO:- The marker is designed for use in each gel lane to increase precision in analyses.
The ILS 600 BIO contains 21 evenly balanced fragments ranging from 80–600bp.
High-Throughput Analysis:- Analysis is achieved by comparison of amplified sample DNA fragments
directly with the allelic ladder present for each locus.
Matrix 16 BIO:- This tool is provided to aid in color separation.
Tolerant:- More tolerant of degraded DNA than other typing methods.
Outstanding Technical Support:- Promega Technical Services Representatives are highly trained scientists
who provide exceptional support for the entire Promega product line.
Promega also provides technical training and
support for its global distribution network and conducts customer
training workshops in human DNA typing.
System Components
- » PowerPlex® 16 BIO 10x Primer Pair Mix
- » PowerPlex® 16 BIO Allelic Ladder Mix
- » Gold ST*R 10X Buffer
- » Bromophenol Blue Loading Solution
- » Gel Tracking Dye
- » 9947A
- » Internal Lane Standard 600 BIO (ILS 600 BIO)
- » Matrix 16 BIO
|
