Genetic Identity Product Profiles: PowerPlex^® Systems

PowerPlex^® 16 System

• Contents
• Description
• Features
• System Components
• Protocol
• FAQ
• Related Information
• Figures
• Catalog Information

Description

The PowerPlex^® 16 System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The System allows the coamplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The PowerPlex^® 16 System contains the loci Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane.

The loci included in the PowerPlex^® 16 System have been selected because they satisfy the needs of several major standardization bodies throughout the world, including the European police network, INTERPOL, the European Network of Forensic Science Institutes (ENFSI), GITAD (Grupo Iberoamericano de Trabajo en Análisis de DNA) and the United States Federal Bureau of Investigation (FBI). The system has undergone extensive validation efforts, including external CODIS validation for database and forensic casework, as well as internal validation. The PowerPlex^® 16 System also contains two new low-stutter, highly polymorphic pentanucleotide repeat loci, Penta E and Penta D. These loci add significantly to the discrimination power of the system, making the PowerPlex^® 16 System a single amplification system with a power of exclusion sufficient to resolve paternity disputes definitively. In addition, the extremely low stutter seen with Penta E and Penta D, makes them ideal loci for evaluation of DNA mixtures often encountered in forensic casework.

Finally, the Amelogenin locus is included in the PowerPlex^® 16 System to allow gender identification of each sample.

The PowerPlex 16 System is designed specifically for use with the Applied Biosystems 3130 and 3130xl Genetic Analyzers, the ABI PRISM 3100 and 3100-Avant Genetic Analyzers, and the ABI PRISM 310 Genetic Analyzer.

Matrix generation is performed using the PowerPlex Matrix Standards, 310 (DG4640) or PowerPlex Matrix Standards, 3100/3130 (DG4650).

Features

• Amplify DNA Once, Not Twice.

• No longer do you have to amplify each database or casework sample twice. With the PowerPlex^® 16 System you can amplify all 13 CODIS STR loci at one time.

• Get the sensitivity you require.

• The PowerPlex^® 16 System provides maximum sensitivity with low quantities of DNA—it is optimized for use with 1ng of DNA. However, each lot is performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with <0.5ng of DNA.

• Find the validation you expect.

• Concordance.

• PowerPlex^® 16 is concordant with Applied Biosystem’s Profiler Plus™, COfiler™ and Identifiler™ PCR Amplification kits.

• Validation Studies.

• Promega’s developmental validation was completed in October 2000. In 2001, more than 20 laboratories participated in a casework validation study to comply with SWGDAM guidelines. Over 2000 samples were typed by multiple CODIS laboratories the required CODIS database validation. Results of these studies are published and are available upon request.

• Realize the speed and simplicity you want.

• Primer Set.

• Consistent balance across loci and between dyes simplifies analysis of low amounts of template DNA and minor contributors of other DNA samples.

• Internal Lane Standard 600.

• This CXR-labeled marker offers the greatest precision available for DNA typing. Peaks range in size from 60–600bp. Fragments that are multiples of 100 bases are present at approximately twice the height of other fragments to simplify size assignment.

• Allelic Ladders.

• Allelic Ladders for all 16 loci are provided to simplify interpretation and improve software compatibility.

• PowerTyper™ Macros.

• The PowerTyper™ Macros has been designed to automatically label fragments generated from GeneScan^® data.

• Outstanding Technical Support.

• Promega’s Forensic Account Managers and Technical Services Representatives are highly trained scientists who provide exceptional support for all genetic identity products. Promega also provides technical training and support for its global distribution network, conducts workshops in human DNA typing, and sponsors symposia on human identification.

System Components

• » PowerPlex^® 16 Primer Pair Mix
• » PowerPlex^® 16 Allelic Ladder Mix
• » Gold ST*R 10X Buffer
• » 9947A DNA
• » Internal Lane Standard 600

Protocol

• » View Protocol Abstract

FAQ

» Open FAQ in new window

Related Information

• PowerPlex^® 16 System Validation
• PowerTyper™ 16 Macro: The PowerTyper™ Macros Release 2.0, has been designed to automatically label fragments generated from GeneScan^® data. The PowerTyper™ Macros can be downloaded from Genetic Identity Downloads or ordered on CD-ROM (Cat.# DG3470).
• The PowerPlex^® 16 System (Profiles in DNA Vol. 4, No.1)
• Megaplex STR Analysis from a Single Amplification: Validation of the PowerPlex^® 16 System (Profiles in DNA Vol. 4, No.2)
• PowerPlex^® 16 Training and Troubleshooting Guide (Profiles in DNA Vol. 4, No.2)
• Krenke, B. et al. (2002) Validation of a 16-Locus Fluorescent Multiplex System. J. Forensic Sci. 47, No. 4. Request Part#: RP067
• Budowle, B. et al. (2001) STR primer concordance study. Forensic Sci. Int. 124, 47–54. Request Part#: RP065
• Budowle, B. and Sprecher, C. (2001) Concordance study on population database samples using the PowerPlex™ 16 kit and AmpFISTR^® Profiler Plus™ kit and AmpFISTR^® COfiler™ kit. J. Forensic Sci. 46, No. 3. Request Part#: RP059
• Amplifications Using the PowerPlex^® 16 System and a 10X Primer Pair Mix Stored in TE^-4 Buffer or in Water Yield Comparable Results (877 kb PDF)
• PowerPlex^® Matrix Standards, 3100
• PowerPlex^® 16 System Brochure

Figures

• » Figure 1. The PowerPlex^® 16 System.
• » Figure 2. Sensitivity of the PowerPlex 16 System.

Catalog Information

» PowerPlex^® 16 System

View Figure 1.

Figure 1. The PowerPlex^® 16 System. A single DNA template (0.5ng) was amplified using the PowerPlex^® 16 10X Primer Pair Mix. The amplification products were mixed with the Internal Lane Standard 600 and run on an ABI PRISM^® 310 Genetic Analyzer using a 3-second injection time. The results were analyzed using GeneScan^® Analysis Software.

• Panel A: An electropherogram showing the peaks of the fluorescein-labeled loci, D3S1358, TH01, D21S11, D18S51 and Penta E.
• Panel B: An electropherogram showing the peaks of the JOE-labeled loci, D5S818, D13S317, D7S820, D16S539, CSF1PO and Penta D.
• Panel C: An electropherogram showing the peaks of the TMR-labeled loci, Amelogenin, vWA, D8S1179, TPOX and FGA.
• Panel D: An electropherogram showing the fragments of the Internal Lane Standard 600.

View Figure 2.

Figure 2: Sensitivity of the PowerPlex^® 16 System. The PowerPlex^® 16 System co-amplifies 15 STR loci and amelogenin from as little as 200pg of DNA. The STR loci, D3S1358, THO1, D21S11, D18S51 and Penta E (shown in blue) are labeled with fluorescein. The STR loci, D5S818, D13S317, D7S820, D16S539, CSF1PO and Penta D (shown in green) are labeled with 6-carboxy-4´, 5´-dichloro-2´, 7´-dimethoxy-fluorescein (JOE). The Amelogenin locus and the STR loci vWA, D8S1179, TPOX, and FGA (shown in black) are labeled with carboxy-tetramethylrhodamine (TMR). The Internal Lane Standard 600 (shown in red) is labeled with carboxy-X-rhodamine (CXR).

• Panel A: 1ng of a single genomic DNA amplified using the PowerPlex^® 16 System and detected using the ABI PRISM^® 310 Genetic Analyzer.
• Panel B: 500pg of a single genomic DNA amplified using the PowerPlex^® 16 System and detected using the ABI PRISM^® 310 Genetic Analyzer.
• Panel C: 200pg of a single genomic DNA amplified using the PowerPlex^® 16 System and detected using the ABI PRISM^® 310 Genetic Analyzer.