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Description
The PowerPlex® 1.2 System provides a rapid, non-radioactive method designed to obtain
a DNA type, or genetic fingerprint using very small amounts (e.g., 1ng) of human DNA.
The PowerPlex® 1.2 System is designed specifically for use with the ABI PRISM® 310
Genetic Analyzer and is also compatible with the ABI PRISM® 373 and 377 DNA Sequencers.
PowerPlex® 1.2 allows for the simultaneous single-tube amplification and two-color
detection of eight polymorphic STR loci. CSF1PO, TPOX, TH01 and vWA primers are
labeled with tetramethylrhodamine; D16S539, D7S820, D13S317 and D5S818 primers
are labeled with fluorescein. All of the loci included in this set are true
tetranucleotide repeats.
To facilitate analysis of the data generated with the PowerPlex® 1.2 System,
we have created a file to allow automatic assignment of genotypes using the
ABI Genotyper® Software. The PowerTyper™ 1.2 Macro is supplied on disk and
facilitates analyzing the PowerPlex® 1.2 data. The PowerTyper™ 1.2 Macro is
available upon request at no additional charge and is also available online.
Features
High-Throughput Analysis:- Analysis is achieved by comparison of amplified sample DNA fragments directly
with the allelic ladder present for each locus.
Size Marker:- Fluorescent Ladder (CXR), 60–400 Bases, is provided with the system and is
tagged with a third fluorescent dye. This marker may be included as an internal
lane standard in each lane to increase precision in analyses when using the PowerPlex® 1.2 System.
Allelic Ladders:- Allelic ladders for all loci are provided to assure balance across loci. This simplifies
interpretation and improves software compatibility.
Efficient:- The PowerPlex® 1.2 System allows simultaneous amplification and analysis of the eight
STR loci plus Amelogenin for gender identification. The Amelogenin system allows
amplification of sequences specific to the X (212-base) and Y (218-base) chromosomes, respectively.
Sensitive:- Amplification of 1–250ng of genomic DNA.
Tolerant:- More tolerant of degraded DNA than other typing methods.
Outstanding Technical Support:- Promega Technical Services Representatives are highly trained scientists
who provide exceptional support for the entire Promega product line.
Promega also provides technical training and
support for its global distribution network and conducts customer
training workshops in human DNA typing.
System Components
- » PowerPlex® 1.2 10X Primer Pair Mix
- » PowerPlex® 1.2 Allelic Ladder Mix
- » Gold ST*R 10X Buffer
- » K562 DNA, High Molecular Weight
- » GenePrint® TH01 Allele 9.3 (TMR)
- » Fluorescent Ladder (CXR), 60–400 Bases
- » Blue Dextran Loading Solution
- » Fluorescein Matrix Standard
- » TMR Matrix Standard
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