|
Description
The PowerPlex® 1.1 System provides a rapid, non-radioactive method designed to obtain
a DNA type, or genetic fingerprint, using very small amounts (e.g., 1ng) of human DNA.
The PowerPlex® 1.1 System allows the co-amplification and two-color detection of eight
STR loci. CSFIPO, TPOX, TH0I and vWA primers are labeled with tetramethylrhodamine;
D16S539, D7S820, D13S317 and D5S818 primers are labeled with fluorescein.
All eight loci are amplified simultaneously in a single tube and analyzed
in a single gel lane. Loci amplified using the PowerPlex® 1.1 System also may
be co-amplified with the Fluorescent Sex Identification System-Amelogenin (TMR),
allowing the detection of nine loci in a single gel lane. The PowerPlex® 1.1 System
is customized for use with the Hitachi FMBIO® and Hitachi FMBIO®
II Fluorescence Imaging Systems.
Features
High-Throughput Analysis:- Analysis is achieved by comparison of amplified sample
DNA fragments directly with the allelic ladder present for each locus.
Size Marker:- Fluorescent Ladder (CXR), 60–400 Bases, is provided with the system. It is tagged with
a third fluorescent dye. This marker may be included as a standard in each gel lane and visualized
separately to monitor fluctuations in sample mobility that may occur from lane-to-lane.
Allelic Ladders:- Allelic ladders for all loci are provided to assure balance across loci.
This simplifies interpretation and improves software compatibility.
Flexible:- The primer sets are compatible with use of either AmpliTaq Gold®
(when used with Gold ST*R 10X Buffer [Cat.# DM2411] sold separately)
or more generic forms of Taq DNA polymerase,
generating few or no amplification artifacts with either.
Sensitive:- Amplification of 1–250ng of genomic DNA.
Tolerant:- More tolerant of degraded DNA than other typing methods.
Outstanding Technical Support:- Promega Technical Services Representatives are highly trained scientists
who provide exceptional support for the entire Promega product line.
Promega also provides technical training and
support for its global distribution network and conducts customer
training workshops in human DNA typing.
System Components
- » PowerPlex® 1.1 Primer Pair Mix
- » PowerPlex® 1.1 Allelic Ladder Mix
- » STR 10X Buffer
- » K562 DNA, High Molecular Weight
- » Bromophenol Blue Loading Solution
- » Gel Tracking Dye
- » TH01 Allele 9.3 (TMR)
- » Fluorescent Ladder (CXR), 60–400 Bases
|
