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Genetic Identity Product Profiles: PowerPlex® Systems

PowerPlex® Matrix Standards, 3100/3130

Description

The PowerPlex® Matrix Standards, 3100/3130, allows Promega's PowerPlex 16 System and PowerPlex Y System to be run on the ABI PRISM® 3100  and 3100-Avant Genetic Analyzers and Applied Biosystems 3130 and 3130xl Genetic Analyzers. Proper generation of a spectral calibration file is critical to evaluate multicolor systems. The PowerPlex Matrix Standards, 3100/3130, contains matrix fragments labeled with the four fluorescent dyes: Fluorescein, JOE, TMR and CXR. Once generated using the PowerPlex dye set, the special calibration file is applied during the analysis of PowerPlex data to calculate and compensate for the spectral overlap between different fluorescent dye colors.

The PowerPlex Matrix Standards, 3100/3130, should be used in combination with the PowerPlex 16 and PowerPlex Y systems.  Users of the PowerPlex ES system should continue to use the PowerPlex Maxtrix Standards, 3100 - Custom (Cat. # X3121).

To generate a matrix on the ABI PRISM 310 Genetic Analyzer, use the PowerPlex Matrix Standards, 310 (Cat. # DG4640).

The PowerPlex Matrix Standards, 3100 (Cat. # DG3650) has been discontinued.

Features

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System Components

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Protocol

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