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Monoplexes
Description
The Fluorescent Monoplex STR Systems have been developed for use with the Hitachi
FMBIO® Fluorescent Scanner, the FMBIO® II Fluorescent Scanner, and the Applied
Biosystems ABI PRISM® 377 DNA Sequencer. One primer for each locus in a multiplex
or monoplex system has been labeled with fluorescein to allow fluorescent
detection. Fluorescein has an excitation maximum at 488nm and a light emission
maximum at 532nm. Therefore, the systems are also compatible with a variety
of fluorescence detection instruments including the Molecular Dynamics FluorImager®
and GenomyxSC™ Fluorescent Scanners, the ABI PRISM® 373 DNA Sequencer, the ABI
PRISM® 310 Genetic Analyzer, and the Pharmacia ALF® (but not the ALFexpress™)
DNA Sequencer.
Features
High-Throughput Analysis:- Analysis is achieved by comparison of amplified sample
DNA fragments directly with the allelic ladder present for each locus.
Convenient:- Results obtained using the CTTv Fluorescent STR System can be recorded in a digitized
format, allowing direct comparison with stored databases.
Sensitive:- Amplification of 1-25ng of genomic DNA.
Tolerant:- More tolerant of degraded DNA than other typing methods.
Allelic Ladder:- Comparison of amplified alleles with the allelic ladders provided with each system
allows rapid and reliable determination of allele content.
Outstanding Technical Support:- Promega Technical Services Representatives are highly trained scientists
who provide exceptional support for the entire Promega product line.
Promega also provides technical training and support for its global
distribution network and conducts customer training workshops in human DNA typing.
System Components
- » STR 10X Primer Pairs (Fluorescein)
- » STR Allelic Ladder (Fluorescein)
- » STR 10X Buffer
- » K562 DNA, High Molecular Weight
- » Bromophenol Blue Loading Solution
- » Blue Dextran Loading Solution
- » Gel Tracking Dye
Figures
No figures are available for this product.
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