Applications of Reporter Gene Assays

Improve Promoter Analysis with Luciferase Reporters

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  • Promoter analysis seeks to understand why a particular gene responds to a particular environmental condition or treatment.
  • Originally, promoter dissection involved deletion analysis of the proximal promoter region until the gene response to the condition or treatment changed or was eliminated.
  • Today, bioinformatics allows researchers to make "educated guesses" about what regions of the promoter will be important
    for a particular response.

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Long Terminal Repeat Promoter Analysis of HIV-1

  • Treating T-cells infected with HIV-1 involves activating the provirus that has integrated into the genome so that these cells can be "marked" for treatment. However, activating the provirus triggers the NF-κB pathway and activates the T-cell, allowing the virus to replicate and infect additional cells. The authors of this study screened for factors that could activate a HIV-1 promoter in which the NF-κB sites were mutated.

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Reporter Assay Applications

Exploring Cellular Signaling with Luciferase Reporters

pGL4 Vectors designed for improved signaling pathway analysis

Analyzing miRNA Targets with Luciferase Reporters

Luciferase reporter designed for miRNA target analysis

Studying Protein Interactions with Luciferase Reporters

Adapting yeast two-hybrid analysis to mammalian systems

Analyzing Nuclear Receptor Signaling with Luciferase Reporters

Luciferase-based one-hybrid system to study nuclear hormone receptor signaling