Reporter Vectors & Cell Lines
Promega offers a variety of vectors for use in genetic reporter assay experiments. Genetic reporters can be used to study biological areas ranging from gene expression and control to cell signaling and drug discovery. The use of luciferase as a reporter provides a system that is extremely sensitive, while also very easy to assay.
Featured Products
pGL4 Luciferase Reporter Vectors
Optimized luciferase reporter vector offers both firefly and Renilla luciferases in a variety of configurations. Now also available with response elements to study pathways of interest.
Go to the pGL4 Vector Selector
pmirGLO Dual-Luciferase miRNA Target Expression Vector
Quantitatively measure microRNA activity with a luciferase reporter.
SwitchGear Genomics Luciferase Reporter Libraries
Ready-to-screen vector libraries developed with SwitchGear Genomics, allow the study of thousands of promoters, UTRs and other regulatory elements across the human genome, using a luciferase reporter gene.
Learn more...Nuclear Receptor Assays
Study nuclear receptors in a universal GAL4 system using an optimized dual-luciferase system.
GloResponse™ Luciferase Reporter Cell Lines
State-of-the-art luciferase reporter technology integrated into a cell line.
pGL3 and pGL2 Luciferase Reporter Vectors
Provide tools for the quantitative analysis of cis- or trans-factors that may regulate mammalian gene expression. The backbone of the pGL2 Vector was redesigned for the pGL3 Vector to increase expression, including a modified firefly (Photinus pyralis) luciferase coding region that has been optimized for monitoring transcription in transfected eukaryotic cells.
More pGL2 and pGL3 Vectors...Chroma-Luc™ Reporter Vectors
Generate red and green (dual-color) luminescence from a single sample upon addition of a single reagent from the Chroma-Glo™ Luciferase Assay System.
pRL Renilla Luciferase Reporter Vectors
Constitutively express Renilla luciferase. May be used with a firefly luciferase vector to cotransfect mammalian cells. Provides an internal control value to normalize experimental firefly luciferase reporter gene expression.
Learn more...Technical Resources
FAQs pGL4 Vector SelectorAnimation
Introduction to Reporter Gene AssaysTools
Chroma-Luc™ CalculatorVectors
Reporters Mammalian ExpressionArticles
Review: David C. Montefiori (2009) Measuring HIV Neutralization in a Luciferase Reporter Gene Assay. HIV Protocols, Second Edition, Vol. 485, V.R. Prasad and G.V. Kalpana, eds. PIASy Regulation of STAT5 Activity: Different Reporter Vectors, Different Responses Next-Generation Assay for Mammalian Protein Interactions: The CheckMate™/Flexi® Vector Mammalian Two-Hybrid System Enhanced Response Dynamics for Transcription Analysis using pGL4 Luciferase Reporter Vectors Normalizing Genetic Reporter Assays: Approaches and Considerations for Increasing Consistency and Statistical Significance pGL4 Vectors: A New Generation of Luciferase Reporter Vectors