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Bright-Glo™ Luciferase Assay System has joined Steady-Glo®
Luciferase Assay System to expand Promega’s family of homogeneous
luciferase assays. Bright-Glo™ Reagent provides at least 4-fold
more light output than other extended half-life luciferase reagents
and up to 10-fold more light output dependent upon cell line and
media used. Signal half-life is approximately 30 minutes, which
is sufficient to read a 96 well plate with less than 5% signal decay
or to perform assays using continuous process automation.
Features:
- High Sensitivity: Most sensitive luciferase reagent
commercially available for homogeneous or non-homogeneous firefly
luciferase assays. Particularly important when studying weak
promoters, when using difficult to transfect cells, or when
using a CCD camera to capture data.
- Scalability: From low- to ultrahigh-throughput applications
using either homogeneous or non-homogeneous assay formats. In
nonhomogeneous format, cells may be lysed with a Promega lysis
buffer prior to assay. Any volume of cell lysate may then be
assayed using an equivalent volume of reagent.
- Homogeneous Assay: Enables rapid quantitation directly
from cells grown in multiwell plates. No need for prior removal
of the culture medium, washing of cells, or preparation of lysates.
- High Assay Precision: Well-to-well variability is
minimized by specialized design features to ensure uniform and
reproducible luminescence within every sample. This is especially
important for optimal performance when using laboratory automation.
- Convenience: Reagent preparation in less than 30
seconds without need for thawing, measuring, or temperature
equilibration. Available in 10ml, 100ml, and 10 x 100ml sizes.
Luminescence profiles for Promega firefly
luciferase assays. Purified luciferase (2.2 x 10-10M)
was quantified in a 96 well plate following the Technical Manual
for each assay reagent. Luciferase was measured from 100µl of Glo
Lysis Buffer for Steady-Glo® and Bright-Glo™ Reagents.
For Luciferase Assay Reagent, luciferase was measured from 20µl
of Cell Culture Lysis Buffer. BSA (1mg/ml) was added to the lysis
buffers as a carrier for the purified luciferase. Luminescent reactions
were initiated by adding 100µl of the respective assay reagents.
Traces represent repeated measurements of a single 96 well plate,
using a 1-second integration per well. Each data point is the average
of 3 wells; relative standard error < 3.3%.
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