Advantages of The Dual-Glo™ Luciferase Assay System

Increased Precision and Accuracy:
Normalize your primary reporter results with an internal control. Co-reporter minimizes effects of cell number, cell health, transfection efficiency and non-specific cellular responses.

Homogeneous Format:
Perform fewer steps. Cells may be assayed directly in growth medium for both reporters. No centrifugation required.

Stable Signal:
Obtain flexibility for either batch or continuous processing of 96, 384 & 1536 well plates. Each luminescent signal may be measured for up to 2 hours after reagent addition.

Convenience:
Screen efficiently with this simple, two-step assay that is ideal for your luminometer. Injectors not required.

Wide Range:
Analyze high and low reporter activity without sample dilution. Linear over at least 6 logs of enzyme concentration for each reporter.

With the Dual-Glo™ Assay, you have a powerful and reliable method to deconvolute your data by enabling you to discriminate between specific and non-specific cellular responses. Single reporter, uncorrected data cannot distinguish between specific down regulation and cytotoxicity, as both responses exhibit a similar decrease in luminescence. In the Dual-Glo ™ Assay, however, the use of dual reporters can discriminate these responses through normalization.

Figure 1. Single Reporter, uncorrected data cannot distinguish specific down regulation from a cytotoxic response: Transiently transfected CHO cells expressing Renilla luciferase under control of the Tet-Off promoter and firefly luciferase under the control of the CMV promoter (luc+ in pCI plasmid from Promega) were exposed to varying concentrations of either Doxycycline (a specific inhibitor that down regulates expression of the Tet-Off promoter) or G418 (a general inhibitor which is cytotoxic to cells). All data was generated using the Dual-Glo™ Luciferase Assay System.

Panel A: Luminescence from the uncorrected experimental reporter, Renilla luciferase, is shown compared to the concentration of the inhibitor Doxycylcine.

Panel B: Luminescence from the uncorrected experimental reporter, Renilla luciferase, is shown compared to the concentration of the general inhibitor G418. The data illustrates that single reporter results cannot distinguish between cytotoxicity and specific down regulation. Non-normalized Reporter CV= 22%.