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News

SwitchGear ready-to-screen vectors representing thousands of promoters, UTRs, and other regulatory elements across the human genome.

Amaxa-Promega Partnership to Enable Simplified Monitoring of Cell Signaling Events
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NEWThe Ultimate in
Reporter Gene Assays

ONE-Glo™ Luciferase Assay Reagent. A new luciferase substrate, creating a reagent that is more stable, more tolerant to sample components and has less odor than standard luciferase assay reagents.

Reporter Vectors

The pGL4 Reporter Vectors
Now featuring vectors with minimal promoters and response elements.

The Chroma-Luc™ Reporter Vectors contain a synthetic version of red or one of two green click beetle luciferase genes for dual-color measurements.

HaloTag™ Interchangeable
Labeling Technology

HaloTag™ Interchangeable
Labeling Technology
bridges cellular imaging and protein analysis with a single tool. A single HaloTag fusion can impart a variety of functionalities, including fluorescence, affinity tagging or direct attachment to a solid surface.

 

Dual Reporter Assays

The Dual-Glo™ Luciferase Assay System is a homogeneous reagent system that enables the fastest and simplest quantitation of co-transfected firefly and Renilla luciferases in microplate formats.

The Chroma-Glo™ Luciferase Assay System is a homogeneous reagent system designed for quantitation of dual-color (red and green) luminescence generated by the Chroma-Luc™ luciferases. Use filters to separate the luminescent signals.

The Dual-Luciferase® Reporter Assay System is a non-homogeneous reagent system that enables the most sensitive quantitation of co-transfected firefly and Renilla luciferases.

Luminometers

The GloMax™ 96 Microplate Luminometer is a state-of-the-art microplate luminometer that meets the needs for high sensitivity and broad dynamic range for all luminescence applications.

The GloMax™ 20/20 Luminometry System is an ultrasensitive, versatile and affordable luminometer designed for use with any Promega bioluminescent assay.

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Look for the DLReady™ Logo when selecting a luminometer to use with Promega's
Dual-Luciferase® Reporter Assay Systems.

Luminometers carrying the DLReady™ logo

Review criteria for the DLReady™ validation program.

Request Literature on DLR.

Resources

View the Introduction to Reporter Gene Assays Animation

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Promega Cell-Based Assays BrochureRequest more information about our bioluminescent cell-based assays and systems...

Single Reporter Assays

 The ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates super bright luminescence for live cell quantitation of Renilla luciferase. Use for kinetic reporter gene analysis, BRET and ddRNAi applications.

The EnduRen™ Live Cell Substrate offers high signal to background ratios and extended signal kinetics for quantitation of Renilla luciferase in live cells. Use for kinetic reporter gene analysis, BRET and ddRNAi applications.

The Bright-Glo™ Luciferase Assay System is a homogeneous reagent system that enables the most sensitive quantitation of firefly luciferase in microplate formats.

Steady-Glo® Luciferase Assay System is a homogeneous reagent system with a signal half-life > 5 hours for quantitation of firefly luciferase in microplate formats.

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Technical Articles

Normalizing Genetic Reporter Assays: Approaches and Considerations for Increasing Consistency and Statistical Significance

Enhanced Response Dynamics for Transcription Analysis Using New pGL4 Luciferase Reporter Vectors

Using Luciferase Reporter Assays to Screen for GPCR Modulators

Using Luciferase Assays to Study G-Protein Coupled Receptor Pathways and Screen for GPCR Modulators

Choosing the Right Luciferase Assay to Light Your Way

See Things in a Brand New Light: Introducing Chroma-Luc™ Technology

Dual-Glo™ Luciferase Assay System: Convenient Dual Reporter Measurements in 96- and 384-well Plates

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