GloSensor™ Technology
New Live-Cell Biosensor for GPCR Studies
The GloSensor™ cAMP Assay provides a simple and powerful new way to measure cAMP levels in cells. cAMP is a key second messenger involved in GPCR signaling, acting through Gs- and Gi-coupled proteins. The assay uses a genetically modified form of firefly luciferase containing a cAMP-binding protein moeity. Binding of cAMP causes a conformational change that leads to increased light output. The assay is well suited to HTS and uHTS platforms.
Download the Technical Update
"GloSensor™ cAMP Assay: Overview & Technical Update, April 2009" (1960 KB PDF)
The Technical Update presents the GloSensor concept, recent performance data with a focus on Gi-coupled receptors and available products.
Product Information
Design Concept for a cAMP Biosensor
The GloSensor™ cAMP Assay is a 'zero step', non lytic, live-cell assay ideal for kinetic and modulation studies of signaling through cAMP. The simple protocol is easily scalable to your throughput needs.
Simple, Scalable Protocol
Pre-equilibrate cells with GloSensor™ cAMP Reagent for ~2 hours.
Treat with specific agonists/antagonists or library compounds.
Measure luminescence after 15-30 minutes.
View complete protocol
Accessing the Technology
The GloSensor cAMP Assay comprises three components of which two are required to perform the assay; the Reagent in combination with the Cell Line or the Plasmid. GloSensor™ cAMP Reagent is required per a specific Limited Use Label License. For more information on licensing GloSensor Technology, please contact us.
References
Binkowski, B., Fan, F., and Wood, K. (2009) Engineered luciferases for molecular sensing in living cells. Curr. Opin. Biotechnol. 20, 1-5.
Fan, F, Binkowski, B et al., (2008) Novel Genetically Encoded Biosensors Using Firefly Luciferase. ACS Chem Biol 3(6) 346-351.
GloSensor is a trademark of Promega Corporation.
Limited Use Label License
Limited Use Label License For research use only subject to the purchaser's prior agreement to the terms of the limited use label license. Use for commercial purposes such as for payment or other consideration, and use in animals each requires additional licensing. This product and/or its use is subject to one or more of the following Promega patent applications: U.S. Pat. Appln. Ser. Nos. 09/645,706, 10/943,508, 10/957,433, 11/316,042, 11/732,105, 11/786,785, 11/825,304, PCT Pat. Appln. Ser. No. PCT/US2007/008176 and various corresponding patent applications and issued patents. .
For additional licensing questions, please contact Promega.
Next Steps
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