GloSensor™ Technology
New Live-Cell Biosensor for GPCR Studies
The GloSensor™ cAMP Assay provides a simple and powerful new way to measure cAMP levels in cells. cAMP is a key second messenger involved in GPCR signaling, acting through Gs- and Gi-coupled proteins. The assay uses a genetically modified form of firefly luciferase containing a cAMP-binding protein moeity. Binding of cAMP causes a conformational change that leads to increased light output. The assay is well suited to HTS and uHTS platforms.
Download the GEN Reprint
"Live-Cell Luminescent Assays for GPCR Studies" (319 KB PDF)
What to Know More? Download the Technical Update
"GloSensor™ cAMP Assay: Overview & Technical Update, November 2009" (985 KB PDF)
The Technical Update presents the GloSensor concept, recent performance data with a focus on Gi-coupled receptors and available products.
Product Information
Design Concept for a cAMP Biosensor
The GloSensor™ cAMP Assay is a 'zero step', non lytic, live-cell assay ideal for kinetic and modulation studies of signaling through cAMP. The simple protocol is easily scalable to your throughput needs.
Simple, Scalable Protocol
Pre-equilibrate cells with GloSensor™ cAMP Reagent for ~2 hours.
Treat with specific agonists/antagonists or library compounds.
Measure luminescence after 15-30 minutes.
View complete protocol
Accessing the Technology
The GloSensor cAMP Assay comprises three components of which two are required to perform the assay; the Reagent in combination with the Cell Line or the Plasmid. GloSensor™ cAMP Reagent is required per a specific Limited Use Label License. For more information on licensing GloSensor Technology, please contact us.
References
Binkowski, B., Fan, F., and Wood, K. (2009) Engineered luciferases for molecular sensing in living cells. Curr. Opin. Biotechnol. 20(1), 14-18.
Fan, F, Binkowski, B et al., (2008) Novel Genetically Encoded Biosensors Using Firefly Luciferase. ACS Chem Biol 3(6) 346-351.
GloSensor is a trademark of Promega Corporation.
Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
NOT FOR USE IN LIVE ANIMALS— For use in live animals contact Promega regarding the availability of licenses. Researchers may use this product and its derivatives in their own research solely for the luminescent determination of cAMP levels in vitro or within cells (excluding uses in living animals). Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence encoding the GloSensor™ cAMP Reporter of this product (i.e., the bioluminescent chimeric protein comprising luciferase and cAMP-binding domains). Researchers may transfer derivatives to others provided that recipients agree to be bound by the conditions of this limited use statement prior to such transfer. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use the GloSensor™ cAMP Reagent purchased from Promega for all determinations of luminescence activity of this product and derivatives; or (2) contact Promega to obtain a license for use of the product and derivatives in conjunction with luminescent assay reagents not purchased from Promega. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
For additional licensing questions, please contact Promega.
Next Steps
Request a Reprint of "Novel Genetically Encoded Biosensors Using Firefly Luciferase" I would like to be contacted by Promega about this new technology. Protocol
FAQs
Commonly asked questions about the GloSensor™ technology