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DNA & RNA Purification

Sample Types Processed Using the SV Total RNA Isolation System
RNA from the following sample types has been successfully purified at Promega or by external laboratories using the SV Total RNA Isolation System.
 

Many of these protocols have been communicated to Promega by external laboratories. Because these protocols have not been performed at Promega, they are intended to be recommendations that might require optimization.

This list will be updated as additional information becomes available. Contact Promega Technical Services (techserv@promega.com) for the latest information on available protocols.

Sample Types Processed Using the SV Total RNA Isolation System.
Sample Type Promega External Comments
Blood and blood cells
  Fresh or frozen whole blood Yes Yes A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
Kephart, D. and Shenoi, H. (1999) Can the SV RNA Isolation System be used to purify RNA from frozen, unprocessed whole blood? enotes
Kephart, D. and Shenoi, H. (1999) Promega Notes 72,
13–4.
Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Hara, N. et al. (2002) Clin. Cancer Res. 8, 1794–9. PubMed ID #12060619
  Human blood plasma and serum Yes Yes A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resource
Fresh or once-frozen human serum (100μl) was added to 175μl of SV RNA Lysis Buffer, then the remainder of the SV Total RNA Isolation System protocol was followed.
Chen, X.Q. et al. (2000) Clin. Cancer Res. 6, 3823–6. PubMed ID #11051224.
  Human leukocytes Yes   A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
  Winter flounder red blood cells   Yes Pedersen, S.F. et al. (2003) Am. J. Physiol. Cell Physiol. 284, C1561–76. PubMed ID #12734109.
  Human peripheral blood mononuclear cells   Yes Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7. PubMed ID #9856816.
Escoffier, C. et al. (1999) J. Virol. 73, 1695–8.
PubMed ID #9882382.
  Primary CD4+ human T lymphocytes   Yes Secchiero, P. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 14620–5. PubMed ID #11114167.
  Viral RNA from human plasma   Yes Personal communication. Contact Promega Technical Services (techserv@promega.com) for more information.
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Sample Type Promega External Comments
Mammalian tissues     A general protocol for total RNA isolation from tissue is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
  Mouse or rat brain Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
Kephart, D. Measuring gene expression from mammalian brain tissue. enotes
Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Kephart, D. and Hooper, K. (1998) Promega Notes 69, 17–9.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse thoracic dorsal root ganglion neurons   Yes Ekström, P.A.R. et al. (2003) Mol. Cell. Neurosci. 22, 383–95. PubMed ID #12691739.
  Mouse central nervous system   Yes Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002. PubMed ID #11106572.
  Mouse small intestine   Yes Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002. PubMed ID #11106572.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82. PubMed ID #10037740.
  Mouse muscle Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82. PubMed ID #10037740.
  Mouse or rat liver Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Sakai, H. et al. (1999) J. Physiol. 519, 323–33.
PubMed ID #10457052.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse kidney Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Attmane-Elakeb, A. et al. (2000) J. Biol. Chem. 275, 33548–53. PubMed ID #10942780.
  Mouse spleen Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002. PubMed ID #11106572.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse embryonic heart, brain and skeletal muscle   Yes Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64. PubMed ID #10744711.
  Mouse or rat lung Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Rat pancreas Yes   Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
  Mouse or rat heart Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse colon   Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse stomach   Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse thymus   Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
  Mouse eyes   Yes Lucas, R.J. et al. (1999) Science 284, 505–7.
PubMed ID #10205062.
  Mouse retina   Yes Freedman, M.S. et al. (1999) Science 284, 502–4.
PubMed ID #10205061.
  Rat whole cochlea, modiola and cochlear sensorineural epithelial/lateral walls   Yes Stöver, T. et al. (2000) Brain Res. Mol. Brain Res. 76, 25–35. PubMed ID #10719212.
  Human muscle   Yes Roth, S.M. et al. (2002) Physiol. Genomics 10, 181–90. PubMed ID #12209020.
  Human placenta   Yes Neuschäfer-Rube, F. et al. (2003) Biochem. J. 371, 443–9. PubMed ID #12519077.
Gonzalez-Perrett, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 1182–7. PubMed ID #11252306.
  Human skin   Yes Yawalker, N. et al. (1999) J. Invest. Dermatol. 113, 43–8. PubMed ID #10417617.
Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7. PubMed ID #9856816.
  Human brain   Yes Miyasaka, T. et al. (2001) Am. J. Pathol. 158, 373–9. PubMed ID #11159174.
  Primary human peritoneal mesothelial cells   Yes Cronauer, M.V. et al. (1999) Am. J. Pathol. 155, 1977–84. PubMed ID #10595927.
  Human cerebrovascular tissue   Yes Peters, D.G. et al. (1999) Nucleic Acids Res. 27, e39. PubMed ID #10572191.
  Human adenoma tissue   Yes Jaquet, P. et al. (2000) J. Clin. Endocrinol. Metab. 85, 781–92. PubMed ID #10690891.
  Human hair follicle Yes   Use fresh samples and at least 10 or more hair roots. Align the hairs, and cut the root end from the hair and quickly process using the SV RNA Isolation System. The amount of RNA is very small and can generally only be detected by RT-PCR. Contact Promega Technical Services (techserv@promega.com) for more information.
  Ferret aorta   Yes Gangopadhyay, S.S. et al. (2002) Biochem. J. 372, 347–57. PubMed ID #12603201.
  Feline oral mucosa   Yes Harley, R. et al. (1999) Clin. Diag. Lab. Immunol. 6, 471–8. PubMed ID #10391845.
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Sample Type Promega External Comments
Plant tissues     A general protocol for total RNA isolation from plant tissue is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
  Tomato leaf Yes   Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
Shenoi, H. and Kephart, D. Titration of tomato leaf material used as starting material for SV total RNA isolation. enotes
Kobs, G. (1998) Promega Notes 68, 28–31.
  Arabidopsis thaliana leaf Yes Yes Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
 Technically Speaking: SV Total RNA Isolation System. Promega Notes 68, 16–9.
Caldelari, D. et al. (2004) Plant Physiol. 126, 1416–29. PubMed ID #11500541.
  Arabidopsis thaliana flower, stem and root   Yes Caldelari, D. et al. (2004) Plant Physiol. 126, 1416–29. PubMed ID # 11500541.
  Tobacco leaf Yes   Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resource
 Technically Speaking: SV Total RNA Isolation System. Promega Notes 68, 16–9.
  Sugar beet 14-day seedling, cotyledon, hypocotyl, 46-day plant and 90-day plant)   Yes Fowler, M.R. et al. (2004) J. Exp. Bot. 353, 2125–6. PubMed ID # 11141186.
  Chicory taproot, etiolated heads, leaf, and green and etiolated seedlings   Yes Bouwmeester, H.J. et al. (2002) Plant Physiol. 129, 134–44. PubMed ID #12011345.
  Three-day-old mung bean hypocotyls   Yes Leiter, H. et al. (1999) J. Biol. Chem. 274, 21830–9. PubMed ID #10419500.
  Pea seedling leaf   Yes Skaf, J.S. et al. (2000) J. Gen. Virol. 81, 1103–9.
PubMed ID #10725438.
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Sample Type Promega External Comments
Cultured cells
(Established cell lines are not listed.)		 Yes   A general protocol for total RNA isolation from mammalian cultured cells is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
  Primary rat endothelial cells   Yes Thum, T., Haverich, A. and Borlak, J. (2000) FASEB J. 14, 740–51. PubMed ID #10744630.
  Fibroblasts derived from mouse embryonic stem cells   Yes Sakai, T. et al. (1998) J. Biol. Chem. 273, 19378–82. PubMed ID #9677354.
  Primary human coronary artery endothelial cells   Yes Zhang, S., Day, I.N.M. and Ye, S. (2001) Physiol. Genomics 5, 187–92. PubMed ID #11328964.
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Sample Type Promega External Comments
Bacteria     A general protocol for total RNA isolation from Gram-positive and Gram-negative bacteria is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
  Escherichia coli Yes Yes Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources
Kobs, G. (1998) Promega Notes 68, 28–31.
Ohmichi, T., Maki, A. and Kool, E.T. (2002) Proc. Natl. Acad. Sci. USA 99, 54–9. PubMed ID #11752404.
  Bacillus subtilis Yes Yes Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
 Additional resources
Kobs, G. (1998) Promega Notes 68, 28–31.
Colombo, M.L. et al. (2004) Antimicrob. Agents Chemother. 48, 484–90. PubMed ID #14742199.
  Vibrio vulnificus   Yes Chen, Y.C. et al. (2004) Infect. Immun. 72, 629–36. PubMed ID #14742502.
  Salmonella enterica   Yes Kelly, A. et al. (2004) Microbiology 150, 2037–53. PubMed ID #15256548.
Klein, J.R., Fahlen, T.F. and Jones, B.D. (2000) Infect. Immun. 68, 3368–76. PubMed ID #10816487.
  Streptomyces coelicolor   Yes The biomass from pregerminated Streptomycetes coelicolor spores was collected, frozen in liquid nitrogen and crushed by mortar and pestle. The resulting powder was processed to extract RNA using the TRIzol® reagent, and the aqueous phase was treated with DNase I. SV RNA Dilution Buffer (375µl) was added to the nucleic acid solution, and samples were mixed and centrifuged. The resulting supernatant was mixed with 250µl of cold 96% ethanol, loaded on an SV spin column and centrifuged for 1 minute. The column was then processed as described in the SV Total RNA Isolation System Technical Manual.
Van Keulen G. et al. (2004) J. Microbiol. Methods 58, 139–42. PubMed ID #15177913.
  Bacillus thuringiensis   Yes Lee, M.K. et al. (2003) Appl. Environ. Microbiol. 69, 4648–57. PubMed ID #12902253.
  Alteromonas   Yes Orikoshi, H. et al. (2003) J. Bacteriol. 185, 1153–60. PubMed ID #12562783.
Tsujibo, H. et al. (2000) Appl. Environ. Microbiol. 66, 3778–83. PubMed ID #10966390.
  Pseudomonas aeruginosa   Yes Li, X-Z., Poole, K. and Nikaido, H. (2003) Antimicrob. Agents Chemother. 47, 27–33. PubMed ID #12499164.
  Yersinia enterocolitica   Yes After resuspension in a lysis buffer (50mM Tris-HCl [pH 7.5], 150mM NaCl), the cell pellet was subjected to French press, and 100μl aliquots were processed with the SV Total RNA Isolation System as directed by the manufacturer. Cambronne, E.D. and Schneewind, O. (2002) J. Bacteriol. 184, 5880–93. PubMed ID #12374821.
  Streptococcus pneumoniae   Yes Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98. PubMed ID #12117949.
  Mycobacterium smegmatis   Yes Milano, A. et al. (2001) J. Bacteriol. 183, 6801–6.
PubMed ID #11698368.
  Klebsiella pneumoniae   Yes Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61. PubMed ID #11257029.
  Rhizobium leguminosarum   Yes Lee, H.Y., An, J.H. and Kim, Y.S. (2000) Eur. J. Biochem. 267, 7224–9. PubMed ID #11106435.
  Pseudomonas putida   Yes Cowles, C.E., Nichols, N.N. and Harwood, C.S. (2000) J. Bacteriol. 182, 6339–46. PubMed ID #11053377.
Grimm, A.C. and Harwood, C.S. (1999) J. Bacteriol. 181, 3310–6. PubMed ID #10322041.
  Rhodopseudomonas palustris   Yes Pelletier, D.A. and Harwood, C.S. (2000) J. Bacteriol. 182, 2753–60. PubMed ID #10781543.
  Neiserria meningitidis   Yes Deghmane, A.E. et al. (2000) EMBO J. 19, 1068–78. PubMed ID #10698947.
  Lactobacillus helveticus   Yes Pederson, J.A. et al. (1999) J. Bacteriol. 181, 4592–7. PubMed ID #10419958.
  Simkania negevensis ZT   Yes Everett, K.D. et al. (1999) J. Bacteriol. 181, 4734–40. PubMed ID #10438738.
  Chlamydia trachomatis   Yes Everett, K.D. et al. (1999) J. Bacteriol. 181, 4734–40. PubMed ID #10438738.
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Sample Type Promega External Comments
Other organisms and tissues
  Saccharomyces cerevisiae Yes   A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
 Additional resource
Kobs, G. (1998) Promega Notes 68, 28–31.
  Gigaspora margarita   Yes Lanfranco, L. et al. (2002) Plant Physiol. 130, 58–67. PubMed ID #12226486.
  Trichomonas vaginalis   Yes Coombs, G.H. et al. (2004) J. Biol. Chem. 279, 5249–56. PubMed ID #14630923.
  Pacific oyster larvae   Yes Steele, S. and Thorndyke, M. (1999) Promega Notes 71,
2–4.
  Cartilaginous fish brain   Yes Valiante, S. and Prisco, M. Detecting pituitary adenylate cyclase-activating polypeptide in cartilaginous fish brain. enotes
  Zebrafish gut   Yes Crosnier, C. et al. (2005) Development 132, 1093–104. PubMed ID #15689380.
  Drosophila larva tissue   Yes Petryk, A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 13773–8. PubMed ID #14610274.
  Frog liver   Yes Saeed-Kothe, A. and Powers-Lee, S.G. (2003) J. Biol. Chem. 278, 26722–6. PubMed ID #12738780.
  Frog gonads and mesonephroi   Yes Ogata, M. et al. (2003) Genetics, 164, 613–20.
PubMed ID #12807781.
  Chicken embryonic heart, brain and skeletal muscle   Yes Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64. PubMed ID #10744711.
  Venom sacs and glands from Naja Atra (Asian cobra)   Yes Lou, X. et al. (2004) J. Biol. Chem. 279, 39094–104. PubMed ID #15252034.
  Microcystis aeruginosa   Yes Kaebernick, M. et al. (2002) Appl. Environ. Microbiol. 68, 449–55. PubMed ID #11823177.
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Sample Type Promega External Comments
Miscellaneous
  In vitro transcribed RNA Yes   Raynor, J.O. and Butler, K.D. (2004) Promega Notes 86, 15–7.
  Human immunodeficiency virus   Yes Personal communication. Contact Promega Technical Services (techserv@promega.com) for more information.
  RNA from soil   Yes Personal communication. Contact Promega Technical Services (techserv@promega.com) for more information.
  MicroRNAs Yes   Total RNA, including microRNA, was isolated from rat liver using the standard tissue protocol in the SV Total RNA Isolation System Technical Manual #TM048. Contact Promega Technical Services (techserv@promega.com) for more information.
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