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MagneSil® ONE, Fixed Yield
Blood Genomic System
If
you are in large-scale clinical or academic genotyping research one of
your major concerns is most likely sample preparation. You need to purify
DNA from a large number of blood or other samples but are spending too
much time performing quantitation and normalization of each DNA sample
prior to amplification.
What you need is speed, but what you really
need is a walk-away method that normalizes DNA yield during purification.
The MagneSil® ONE, Fixed Yield Blood Genomic System frees up your
time.
The MagneSil® ONE, Fixed Yield Blood Genomic System
is a high-throughput system for DNA purification from blood. The system is designed to purify a “fixed yield” of
1µg of DNA (+/-50%) from 50-60ul of anti-coagulated liquid blood in a 96-well walk-away automated format. Set up and place the blood samples on the instrument and start the instrument. That’s it. A Beckman Coulter Biomek® FX robot will complete purification in about 1 hour.
| Yield (ng) |
A260/A280 |
| 935 |
1.91 |
| 799 |
2.14 |
| 1030 |
1.91 |
| 916 |
1.96 |
| 1077 |
1.92 |
| 829 |
1.88 |
| 871 |
1.75 |
| 864 |
1.96 |
| 817 |
1.57 |
| 871 |
1.97 |
| 950 |
1.95 |
| 1079 |
1.76 |
Example yield from 12 blood donors, 4 replicated
samples. Average yield is 919ng with an average A260/A280 ratio of 1.89.
Purification is accomplished using Promega’s MagneSil®- Fixed Yield paramagnetic particles, optimized buffers, and the Deep Well MagnaBot® Magnetic Separation Stand. The procedure consists of three simple steps.
- Whole blood is added to Lysis Buffer and MagneSil particles and is mixed to lyse white cells and bind DNA to the particles.
- Washes are done to remove heme, protein and other contaminants.
- Finally, a fixed yield of DNA is eluted in Elution Buffer ready to be analyzed by PCR, multiplex PCR, or other genotyping applications.
Multiplex analysis of wild type male DNA.
Lanes 2-7 show amplification products from reactions using Multiplex A
Master Mix with male DNA.
Benefits:
- Achieve Consistent Results: Obtain 1μg (fixed yield) of highly pure DNA from 60μl of blood.
- Perform Less Steps: Eliminate DNA quantitation prior to PCR.
- Save Time: Complete walkaway automation available.
Catalog Information
MagneSil® ONE,
Fixed Yield Blood Genomic System
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