Cloning & DNA Markers
Laugh Your Way Through the Lab with Tech Toons!
"Tech Toons" contains handy technical tips on PCR optimization, T-vector cloning and routine subcloning as well as cartoons to brighten your day and make learning fun.
Featured Applications
Flexi® Cloning System
Rapid, high-fidelity transfer of protein-coding regions between vectors.
Many vectors for different applications
(bacterial, mammalian, or cell-free protein expression,
imaging or protein purification).
PCR Cloning
Four simple steps for PCR cloning, without the hassle, steps and expense of other methods.
- Amplify: How does your Taq compare to GoTaq? Take the GoTaq Challenge and find out.
- Purify: Purify your PCR product from a gel slice or directly from PCR in 15 minutes using the
Wizard® SV Gel and PCR Clean-Up System. - Clone: Clone your PCR product into the pGEM®-T Vector of your choice in about an hour.
- Transform: Transform Competent cells, use Promega's High Efficiency JM109, or Single-Step (KRX) competent cells.
Cloning Resource Guide
The cloning chapter of the Protocols and Applications Guide covers cloning using PCR and restriction enzymes, including DNA clean-up, ligation, transformation and screening to identify recombinant clones.
Restriction Enzymes Resource Guide
The Restriction Enzymes Resource Guide contains useful interactive searches and reference information including:
- Information on specific enzymes, enzymes generating compatible ends, enzymes by recognition sequence, and suitable buffers for double-digests.
- Restriction Enzyme Reaction Buffer Composition
- Restriction Enzyme Activity in Promega 10X Buffers
- Heat inactivation and methylation sensitivity of Promega restriction enzymes