Cloning & DNA Markers

Tech Toons Cartoon Book

Laugh Your Way Through the Lab with Tech Toons!

"Tech Toons" contains handy technical tips on PCR optimization, T-vector cloning and routine subcloning as well as cartoons to brighten your day and make learning fun.

Featured Applications

Flexi® Cloning System

Rapid, high-fidelity transfer of protein-coding regions between vectors.

Many vectors for different applications
(bacterial, mammalian, or cell-free protein expression, imaging or protein purification).

See Catalog listing View Flexi Vector Features Compare to Gateway Cloning....

PCR Cloning

Four simple steps for PCR cloning, without the hassle, steps and expense of other methods.

  1. Amplify: How does your Taq compare to GoTaq? Take the GoTaq Challenge and find out.
  2. Purify: Purify your PCR product from a gel slice or directly from PCR in 15 minutes using the
    Wizard® SV Gel and PCR Clean-Up System.
  3. Clone: Clone your PCR product into the pGEM®-T Vector of your choice in about an hour.
  4. Transform: Transform Competent cells, use Promega's High Efficiency JM109, or Single-Step (KRX) competent cells.

DNA Markers

DNA Step Ladders BenchTop DNA Markers Conventional DNA Markers

Cloning Resource Guide

The cloning chapter of the Protocols and Applications Guide covers cloning using PCR and restriction enzymes, including DNA clean-up, ligation, transformation and screening to identify recombinant clones.

Visit the Protocols and Applications Guide..

Restriction Enzymes Resource Guide

The Restriction Enzymes Resource Guide contains useful interactive searches and reference information including: