Cloning & DNA Markers
Flexi® Cloning System
Rapid, high-fidelity transfer of protein-coding regions between vectors.
Many vectors for different applications (bacterial, mammalian, or cell-free protein expression, imaging and protein purification).
See How the Flexi System Works Flexi Vector Selector View Flexi Vector Features Compare to Gateway Cloning How to use the Flexi VectorsSee Catalog listingFeatured Cloning Applications
T-Vector Cloning: See how easy it is!
Restriction Enzyme Resources from Promega
Recognition sequences Compatible ends Find an enzyme Find buffers for double-digests Reaction Buffer Composition Restriction Enzyme Activity in Promega 10X Buffers, Optimal Reaction Temperature and Heat inactivation Methylation sensitivitiesCloning Resource Guide
The cloning chapter of the Protocols and Applications Guide covers cloning using PCR and restriction enzymes, including DNA clean-up, ligation, transformation and screening to identify recombinant clones.