Cloning
Increase Your Productivity with Promega Cloning Products
Promega offers a complete line of cloning products, including restriction enzymes, DNA and RNA modifying enzymes (polymerases, ligases, alkaline phosphatases), DNA markers, vectors, and more.
Featured Applications
Flexi® Cloning System
Rapid, high-fidelity transfer of protein-coding regions between vectors.
Many vectors for different applications
(bacterial, mammalian, or cell-free protein expression,
imaging or protein purification).
PCR Cloning
Four simple steps for PCR cloning, without the hassle, steps and expense of other methods.
- Amplify: How does your Taq compare to GoTaq? Take the GoTaq Challenge and find out.
- Purify: Purify your PCR product from a gel slice or directly from PCR in 15 minutes using the
Wizard® SV Gel and PCR Clean-Up System. - Clone: Clone your PCR product into the pGEM®-T Vector of your choice in about an hour.
- Transform: Transform Competent cells, use Promega's High Efficiency JM109, or Single-Step (KRX) competent cells.
Single Step Competent Cells
Single Step (KRX) Competent Cells can be used for cloning and screening, and allow tightly controlled protein expression. Find out more in the following articles:
The Single Step (KRX) Competent Cells: Efficient Cloning and High Protein Yields Protein Expression in Less Time: A Short Induction Protocol for KRXCloning Resource Guide
The cloning chapter of the Protocols and Applications Guide covers cloning using PCR and restriction enzymes, including DNA clean-up, ligation, transformation and screening to identify recombinant clones.
Restriction Enzymes Resource Guide
The Restriction Enzymes Resource Guide contains useful interactive searches and reference information including:
- Information on specific enzymes, enzymes generating compatible ends, enzymes by recognition sequence, and suitable buffers for double-digests.
- Restriction Enzyme Reaction Buffer Composition
- Restriction Enzyme Activity in Promega 10X Buffers
- Heat inactivation and methylation sensitivity of Promega restriction enzymes