Featured Products

Multiplexed Assay to determine Mechanism of Cell Death

cytotoxicity, viability and apoptosis detection with a luminescent apoptosis assay The ApoTox-Glo™ Assay is designed to provide predictive mechanism of toxicity determination with a simple and fast in vitro assay that measures 3 key independent biomarkers for viability, cytotoxicity and apoptosis in the same sample well of cells.

 

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Homogeneous Luminescent Caspase Assays

Luminescent assays have a much greater linear dynamic range than fluorescent assays.The Caspase-Glo® Assays monitor toxic effects of compounds at high-throughput capacities, or one sample at a time, in as little as 15 minutes.


Caspase-Glo® 2 Assay... Caspase-Glo® 6 Assay... Caspase-Glo® 3/7 Assay... Caspase-Glo® 8 Assay... Caspase-Glo® 9 Assay...

Homogeneous Fluorescent Caspase-3/7 Assay

The Apo-ONE® Homogeneous Caspase-3/7 Assay is a fast, sensitive, fluorescent apoptosis detection system featuring a homogeneous format.

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Nonhomogeneous Caspase Assays

The CaspACE™ Assay System, Colorimetric, provides a colorimetric substrate and a cell-permeable inhibitor that allow highly sensitive, quantitative measurement of caspase-3 (DEVDase) protease activity, an early regulatory event in the apoptotic cell death process.

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The CaspACE™ Assay System, Fluorometric, provides fluorogenic substrates and inhibitors that allow highly sensitive, quantitative measurement of both ICE (Caspase-1) and CPP32 (Caspase-3) protease activities. This assay system may be used with purified enzyme preparations or cell extracts and also can be adapted for use in high throughput systems.

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In Situ Caspase Assay

CaspACE™ FITC-VAD-FMK In Situ Marker is a fluoroisothiocyanate (FITC) conjugate of the cell-permeable caspase inhibitor VAD-FMK. This structure allows delivery of the inhibitor into the cell where it binds to activated caspase, serving as an in situ marker for apoptosis. The bound marker is localized by fluorescence detection.

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TUNEL Assays

DeadEnd™ Fluorometric TUNEL System DeadEnd™ Colorimetric TUNEL System

Caspase Inhibitors

Z-VAD-FMK is a cell-permeant pan caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases, inhibiting induction of apoptosis.

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Ac-DEVD-CHO is an inhibitor of caspase-3/7 (DEVDase) activity.

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Apoptosis-Related Antibodies

Detect the cleaved form of PARP, active caspase-3 or phosphorylated kinases with proven antibodies for imaging and western blotting applications.

Anti-pS473 Akt pAb... Anti-ACTIVE® Caspase-3 pAb Anti-PARP p85 Fragment pAb

Luminescence Detection

GloMax™ Integrated Luminescence Systems are high performance luminometers designed to function seamlessly with Promega luminescence assays. Promega also provides DLReady certification of luminometers compatible with the Dual-Luciferase® Assay.

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