Apoptosis

Morphological changes during apoptosis.We offer an extensive array of apoptosis assay systems and detection reagents, including luminescent and fluorescent caspase assays and antibodies, for the measurement of early or late events in apoptosis.

Featured Products

Homogeneous Luminescent Caspase Assays

Following Caspase-9 cleavage a substrate for luciferase is released, a luciferase reaction occurs and light is produced.The Caspase-Glo® Assays monitor toxic effects of compounds at high-throughput capacities, or one sample at a time, in as little as 15 minutes.




Caspase-Glo® 2 Assay... Caspase-Glo® 6 Assay... Caspase-Glo® 3/7 Assay... Caspase-Glo® 8 Assay... Caspase-Glo® 9 Assay...

Homogeneous Fluorescent Caspase-3/7 Assay

The Apo-ONE® Homogeneous Caspase-3/7 Assay is a fast, sensitive, fluorescent apoptosis detection system featuring a homogeneous format.

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Nonhomogeneous Caspase Assays

The CaspACE™ Assay System, Colorimetric, provides a colorimetric substrate and a cell-permeable inhibitor that allow highly sensitive, quantitative measurement of caspase-3 (DEVDase) protease activity, an early regulatory event in the apoptotic cell death process.

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The CaspACE™ Assay System, Fluorometric, provides fluorogenic substrates and inhibitors that allow highly sensitive, quantitative measurement of both ICE (Caspase-1) and CPP32 (Caspase-3) protease activities. This assay system may be used with purified enzyme preparations or cell extracts and also can be adapted for use in high throughput systems.

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In Situ Caspase Assay

CaspACE™ FITC-VAD-FMK In Situ Marker is a fluoroisothiocyanate (FITC) conjugate of the cell-permeable caspase inhibitor VAD-FMK. This structure allows delivery of the inhibitor into the cell where it binds to activated caspase, serving as an in situ marker for apoptosis. The bound marker is localized by fluorescence detection.

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DeadEnd™ Fluorometric TUNEL System

Ideal for non-radioactive, simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level or in cell suspensions, this system measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3ยด-OH DNA ends, which can then be visualized by fluorescence microscopy or quantitated by flow cytometry.

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DeadEnd™ Colorimetric TUNEL System

Provides simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level. The system measures nuclear DNA fragmentation, an indicator of apoptosis. The system can be used to assay apoptosis in both tissue sections and cultured cells.

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Caspase Inhibitors

Z-VAD-FMK is a cell-permeant pan caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases, inhibiting induction of apoptosis.

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Ac-DEVD-CHO is an inhibitor of caspase-3/7 (DEVDase) activity.

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Apoptosis-Related Antibodies

Anti-pS473 Akt pAb... Anti-ACTIVE® Caspase-3 pAb Anti-Cytochrome c mAb Anti-PARP p85 Fragment pAb