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The CellTiter-Glo® Luminescent Cell Viability Assay provides
a homogeneous bioluminescent assay designed for cytotoxicity and cell proliferation
studies that yields a rapid, simple and sensitive determination of the number of viable cells in culture.
ADVANTAGES
Sensitive
Detects <10 cells per well in a 384-well format.
Detects as few as 50 cells per well in a 96-well format.
Accurately measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.
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| Increased sensitivity reduces the number of cells required per assay, a plus where cells are at a premium. |
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Simple
The homogeneous "add-mix-and-measure" format (single-step addition of Reagent) results in cell lysis and generation of a luminescent signal
proportional to the amount of ATP present in cells.
No cell washing, removal of medium or multiple pipetting steps are required.
Dramatically reduces plate-handling steps required for similar assays. |
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Fast
Data can be recorded 10 minutes after addition of reagent.
Eliminates the need for hours of incubation required with other assays, thus decreasing throughput time.
Flexible
Can be used with various multi-well plate formats. |
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| Data can be recorded by luminometer or CCD camera imaging device.
Robust
Luminescent signal is very stable, with a half-life generally >5 hours, depending on cell type and medium used.
The robustness of the assay can also eliminate the need for
reagent injectors. |
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Application: Cell Viability
Classic cell viability assays such as [3H]Thymidine incorporation assays,
tetrazolium-based colorimetric assays using MTT or MTS,
resazurin, or assays that detect lactate dehydrogenase
(LDH) are commonly used for growth factor assays, optimization of culture conditions,
determining the results of apoptosis, drug screening, and toxicity
screening. The innovative CellTiter-Glo® Assay can be used for all of
these cell viability applications. The assay generates a “glow”
luminescent signal in the presence of ATP from viable cells, which is detected with a
plate reader luminometer or CCD imaging device. The procedure involves the simple addition of a
single reagent directly to cells cultured in serum-supplemented medium.
Application: Cytotoxicity
No other method combines the sensitivity, ease and accuracy of the CellTiter-Glo® Luminescent Cell Viability Assay.
The one-step homogeneous protocol of the CellTiter-Glo® Assay enables laboratories to
precisely monitor toxic effects of compounds at any throughput capacity. With a sensitivity of 15 cells per well, and a one-step homogeneous
method, tens of thousands of compounds can be screened
per day. |
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| Product Information |
Size |
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Cat.# |
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CellTiter-Glo® Luminescent
Cell Viability Assay |
10ml |
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G7570 |
Each vial of substrate is sufficient for 100 assays in 96-well format or 400 assays in
384-well format. Includes:
• 1 x 10ml CellTiter-Glo® Buffer
• 1 vial CellTiter-Glo® Substrate (lyophilized) |
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CellTiter-Glo® Luminescent
Cell Viability Assay |
10 x 10ml |
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G7571 |
Each vial of substrate is sufficient for 100 assays in 96-well format or 400 assays in
384-well format. (1,000-4,000 total assays). Includes:
• 10 x 10ml CellTiter-Glo® Buffer
• 10 vials CellTiter-Glo® Substrate (lyophilized) |
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CellTiter-Glo® Luminescent
Cell Viability Assay |
100ml |
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G7572 |
Each vial of substrate is sufficient for 1,000 assays in 96-well format or 4,000 assays in
384-well format. (1,000-4,000 total assays). Includes:
• 1 x 100ml CellTiter-Glo® Buffer
• 1 vial CellTiter-Glo® Substrate (lyophilized) |
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CellTiter-Glo® Luminescent
Cell Viability Assay |
10 x 100ml |
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G7573 |
Each vial of substrate is sufficient for 1,000 assays in 96-well format or 4,000 assays in
384-well format. (10,000-40,000 total assays). Includes:
• 10 x 100ml CellTiter-Glo® Buffer
• 10 vials CellTiter-Glo® Substrate (lyophilized) |
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