CellTiter-Blue® Cell Viability Assay is the flexible choice in a
homogeneous method for estimating the number of viable cells present
in multiwell plates.
This easily automated assay is flexible and allows you many choices:
- Choice between fluorometric or colorimetric detection methods
- Choice between 96- or 384-well formats
- Opportunity to multiplex assays
- Choice of the duration of incubation to maximize sensitivity.
The assay procedure involves addition of a single reagent (CellTiter-Blue® Reagent) directly to cells cultured in 96- or 384-multiwell plates,
incubation for 1-4 hours, and recording fluorescence or absorbance.
The signal produced by conversion of resazurin to resorufin is
directly proportional to viable cell number.
Features:
Saves you time: the homogeneous add-incubate-measure format
reduces the number of handling steps as compared to radioactive [3H]thymidine
incorporation assays or MTT assays.
Figure 1. The CellTiter-Blue® Cell
Viability Assay protocol. Multiwell plates (96- or 384-well)
compatible with fluorescent plate readers are prepared with cells
and the compounds to be tested. CellTiter-Blue® Reagent is added
directly to each well (20µ reagent to each 100µ of medium in 96-well
format), the plates are incubated at 37°C, and the fluorescent
signal is measured.
Allows you to choose an assay format and method of detection:
Can be used with 96- or 384-well formats and data can be recorded
using fluorescence (preferred) or absorbance.
Convenient-whatever your throughput needs: The reagent
has been designed to provide sufficient volumes for accurate
pipetting into 96- or 384-well formats. Convenient product sizes
available for high-throughput screening.
Get more information from each sample: Multiplex the assay
with Promega's Apo-ONE® Homogeneous Caspase-3/7 Assay
(Cat.# G7790) for viability determination and caspase activation.
Figure 2. Multiplexing two assays
in the same well. Jurkat cells were treated with various
concentrations of staurosporine. CellTiter-Blue® Reagent was added
to each well immediately after drug addition, and the cells were
incubated for 5 hours prior to recording fluorescence (560Ex/590Em).
The caspace activity was measured in the same wells by adding 120µ
of the Apo-ONE® Homogeneous Caspace-3/7 Assay
Reagent. Cells were incubated for an additional hour at ambient
temperature prior to recording fluorescence (485Ex/527Em).
Get the results you need: The Assay uses highly purified
resazurin optimized for robust and sensitive detection. The
optimized assay results in a greater fluorescent signal and reduced
fluorescent background compared to other resazurin-based assays,
allows flexibility in the duration of incubation for maximizing
sensitivity, and achieves highly reproducible results with an
excellent Z′ factor.
Figure 3. Data points used to
obtain Z′ factor value for 500 cells at 4 hours. Fluorescence
after 4 hours incubation at 37°C with CellTiter-Blue® Reagent is
shown for all 96 data points for 0 and 500 L929 cells/well using a
384-well plate. The black line represents the mean for that series;
red lines above and below represent 3 standard deviations of the
mean. A Z′ factor value of 0.80 was achieved under these conditions.
View Online Catalog Information
CellTiter-Blue® Cell Viability Assay |
