Wizard® MagneSil™ Sequencing Reaction Clean-Up System
The Wizard®
MagneSil™ Sequencing Reaction Clean-Up System is a high-throughput solution to
sample purification. The system is designed to purify BigDye™ terminator cycle
sequencing reactions to ensure optimal fluorescent sequencing results. The system
removes buffer salts and unincorporated dye-labeled terminators from sequencing extension
products prior to loading onto a fluorescent instrument. The procedure can be
performed in a standard 96-well PCR plate and may be processed on a robotic workstation.
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Clean up is performed using the MagnaBot® II Magnetic
Separation Device. It is shown here with the Plate Clamp 96.
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Clean up is peformed using Promega's
unique MagneSil™ GREEN paramagnetic particles and the MagnaBot® II
Magnetic Separation Device. This innovative device is designed to work with most
robotic platforms.
The procedure consists of an easily
automated three-step protocol. MagneSil™ particles selectively bind to
sequencing extension products. Non-specifically adsorbed material is washed away
with ethanol. The purified sequencing extension products are eluted in gel loading
solution and are ready to be analyzed by high-throughput DNA sequencing.
FEATURES
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Utilizes
MagneSil™ Magnetic Particle Technology
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DNA capture is performed using paramagnetic particles. No
centrifugation or vacuum required. |
Fully
Automatible
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Process multiple plates with no user intervention. |
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MagneSil™ systems undergo functional testing to ensure
performance in fluorescent capillary and gel electrophoresis sequencing systems. The
system is qualified to produce a lock on position of less than or equal to 25 bases. |
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Sequencing data quality meets or exceeds that obtained with gel
filtration or ethanol precipitation methods. |
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Typically, Phred 20 values for sequencing reactions purified with
the MagneSil™ Sequencing Reaction Clean-Up System are greater than or equal to 600
bases. |
DATA
QUALIFIED
FOR FLUORESCENT SEQUENCING
Electropheragram
of purified sequencing reactions analyzed on an ABI PRISM® 3700 DNA Sequencer.
Reactions
purified with the Wizard®
MagneSil™Sequencing Reaction Clean-Up System.
The
MagneSil™ Sequencing Reaction Clean-Up System purifies sequencing reaction extension
products generated from a variety of template DNAs.
CONSISTENT
PERFORMANCE
Percent
accuracy of sequencing data from 96 samples purified using the MagneSil™
Sequencing Reaction Clean-Up System. Samples were
analyzed in a 96-well plate on an ABI PRISM® 3700 DNA Sequencer and purified
using the Wizard® MagneSil™Sequencing Reaction Clean-Up System.
The MagneSil™ Sequencing Reaction Clean-Up System
offers the distinct advantage of a hands-off protocol while achieving sequencing quality
that is superior to reactions purified by gel filtration or ethanol precipitation.
The system undergoes rigorous testing for performance to ensure it can meet or exceed 98%
accuracy out to 600 bases of sequence and routinely achieves 98% accuracy out to 700
bases.
REPRODUCIBLE RESULTS
Phred 20 values for
sequencing data from each well of a 96-well plate using pGEM®
and MagneSil™ Sequencing Reaction Clean-Up System.
Wizard and MagnaBot are trademarks
of Promega Corporation and are registered with the U.S. Patent and Trademark Office.
MagneSil is a trademark of Promega Corporation.
BigDye is a trademark and ABI PRISM is a registered trademark of Applied Biosystems, a
business of Applera Corporation.
Biomek 2000 is a registered trademark of
Beckman Coulter, Inc.
Products may be covered by pending or
issued patents, please see our Patent Information
for current status.
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