Wizard® MagneSil™ Plasmid Purification
System
The Wizard®
MagneSil™ Plasmid Purification System offers a cost-effective method for purifying
high-quality plasmid DNA. The system is designed exclusively for use with automated
instruments allowing truly "walkaway" high-throughput plasmid purification.
DNA isolation is performed using
Promega's unique MagneSil™ Paramagnetic Particles and a magnetic separation device,
such as the MagnaBot® 96 well Magnetic Separation Device, which is adaptable to any
robotic platform. The protocol has also been optimized using an orbital shaker, such
as the Micro Mix 5 orbital shaker with fitted platform and plate holders
(Cat.# G5016 MM5
kit, Acme Automation, www.acme-automation.com).
MagneSil™ Paramagnetic Particles are used in
separate steps to clear bacterial lysates (MagneSil™ BLUE) and to capture plasmid DNA
(MagneSil™ RED). The use of particles circumvents the need for centrifugation or
vacuum manifolds, making the system ideal for automation. Eluted DNA is ready for use in
fluorescent sequencing reactions as well as other standard molecular biology
techniques. Protocols are available for
plasmid purification in a 96 well format.
FEATURES
Utilizes MagneSil™ Magnetic Particle
Technology
Lysate clearing and DNA capture is performed using magnetic particles. No
centrifugation or vacuum required.
Fully
Automated
Process multiple plates with no user intervention.
Qualified for Fluorescent Sequencing
MagneSil™ plasmid systems undergo functional testing to ensure performance in
capillary electrophoresis
fluorescent sequencing systems.
The Wizard® MagneSil™ Plasmid Purification System purifies high-quality plasmid DNA for use in a number of molecular biology applications including
fluorescent sequencing. The system undergoes rigorous testing for performance in dye
terminator sequencing to ensure it can meet or exceed 600 bases with greater than 98%
accuracy.
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Figure 1. Accuracy by read length for plasmid DNA sequenced following isolation by
MagneSil™ Paramagnetic Particles.
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Consistent Recovery
Reliable recovery of plasmid DNA is ensured. A number of factors including plasmid copy number and cell
density affect the final yield of plasmid DNA. Another important factor is how
consistent the system is at purifying multiple samples. The MagneSil™ Plasmid
Purification System has been tested at a number of cell densities to ensure that DNA
recovery is reliable across multiple wells of a 96 well plate as well as between plates.
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Figure 2. Plasmid
DNA was purified from multiple samples grown to 4 O.D.600. DNA yield was measured
on an agarose gel.
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Reproducible Results
MagneSil™ products can be relied upon to purify high-quality template for fluorescent
sequencing. High-throughput sequencing requires a
reliable purification system. The Wizard® MagneSil™ Plasmid Purification System can be
relied on to produce high-quality templates time and again to ensure accurate sequencing.
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Figure 3. 3 x 96 well
plates (288 samples) containing identical cell preps
(DH5, containing pGEM®-3Zf(+)) were prepared using the
MagneSil™ Plasmid Purification System. Samples were sequenced on an ABI Model
3700 Sequencer.
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Flexible
Format
Complete systems or individual components allow customization to suit individual
needs.
Wizard, pGEM and MagnaBot are
trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark
Office. MagneSil is a trademark of Promega Corporation.
BigDye is a trademark of The Perkin-Elmer Corporation.
Biomek 2000 is a registered trademark of Beckman Coulter, Inc.
Products may be covered by pending or
issued patents. Please visit our Patent Information
for more information.
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