Promega

ApoTox-Glo™ Triplex Assay

Predictive Mechanism of Toxicity Determination

Measure viability, cytotoxicity, and apoptosis in the same sample well:

Accurately determine mechanism of cell death in the same sample well, save time and use less cells.

Easily implement:

The assay follows a simple sequential "add-mix-measure" protocol.

Normalize data with a built-in control:

The ratio of the number of live cells/number of dead cells is independent of cell number and normalizes data making it more comparable well-to-well, plate-to-plate, and day-to-day.

Flexible and easily automated:

Component volumes can be scaled to meet throughput needs and reagents are robust for automation in 96- to 1536-well plate formats.

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Predictive Mechanism of Toxicity Determination

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Measure viability, cytotoxicity and apoptosis in the same sample well

Primary Necrosis

Ionomycin treatment of Jurkat cells for 6 hours.

  • Dose-dependent decrease in viability
  • Increase in cytotoxicity
  • No caspase-3/7 activity

Apoptosis

Staurosporine treatment of Jurkat cells for 6 hours.

  • Dose-dependent decrease in viability
  • Increase in cytotoxicity
  • Increase in caspase-3/7 activity

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Easily implement and adapt to multiwell formats

Simple add-mix-measure protocol and scalable assay reagents make the assay easy to implement and adaptable for 96- to 1536-well formats.

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Normalize data

The ratio of number of live cells vs. number of dead cells is independent of cell number and normalizes the data. This normalization makes results more comparable well-to-well, plate-to-plate and day-to-day.

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