Pull-down Assays
Quickly characterize multiple protein:protein interactions by expressing the protein of interest as a fusion protein (typically in E. coli) and the potential interacting prey(s) using TnT® Systems.
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Co-ImmunoprecipitationAvoid transfection and the generation of cell lysates by expressing both the bait and prey proteins in TnT® Systems and then use appropriate antibodies for immunoprecipitation.
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Electrophoretic Mobility Shift Assay (Gel Shift)Complement other techniques used to analyze protein:nucleic acid interactions by expressing protein in TnT® Systems. Incubate with labeled DNA/RNA fragment and observe migration patterns through a non-denaturing polyacrylamide gel.
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