ReliaPrep™ RNA Cell Miniprep Systems
Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.
The new ReliaPrep™ RNA Cell Miniprep System recovers RNA from precious samples containing as few as 100 cells and allows elution in 15µl or less providing concentrated, pure RNA ready for analysis.
- Process from 100 – 5 million cells
- Elute in 15µl or less
- Generate RNA free of inhibitors
View data generated by other scientists, try ReliaPrep RNA purification and share your data for a chance to receive a discount on RNA products from Promega.
“A very good kit for extracting total RNA from a very small amount of starting material”
“What I love best is the small elution volume, before I had to use RNA concentrator columns on my purified RNA”
Shared data!
RNA was isolated in parallel from 100,000 HeLa Cells. RNA was eluted in various volumes from 7µl to 20µl. For 10 and 7µl elutions, the initial elute was re-applied to the columns and passed through the membrane a second time allowing more complete recovery of RNA and higher concentration. The % recovery is normalized to the recommended elution volume of 15µl.
RNA was isolated in duplicate experiments and analyzed using an Agilent Bioanalyzer. Data is representative of traces obtained. RNA obtained using the ReliaPrep™ RNA Cell Miniprep System typically exhibit RIN values of 8.5 to 10.
RNA was isolated from decreasing numbers of HeLa cells with ReliaPrep™ RNA Cell Miniprep System or a competing system. RNA was quantitated using a qPCR-based quantitation method.
RNA was purified in parallel from various numbers of HeLa cells either with a competing method or with the ReliaPrep™ RNA Cell Miniprep System. RNA was added to TaqMan RT-1PCR assays adding from to 9.5µl of RNA. Following amplification, the qPCR Cq data was analyzed to determine relative concentration for all amplifications. The data was then normalized to the 1µl input sample data for comparison. Green = 90-100%, Yellow = 80-90% and Red = <80% of the 1µl sample.
