Overview

What is Identity Automation™?

Identity Automation™ solutions are optimized automation packages for DNA-based human identification. Packages are currently available for the Differex™ System (differential extraction), the DNA IQ™ System (extraction), the Plexor® HY System setup (quantification) and the Normalization and PowerPlex® setup (STR analysis) on the robotic platforms listed below.

Packages include:

  • Automated methods/scripts
  • Promega hardware & accessories
  • Forensic-tailored user interfaces
  • Installation by a chemistry-knowledgeable scientist
  • Adaptation of methods to desired labware, layout, and workflow
  • Staff training in parallel with installation
  • On-going chemistry and automation support from one company

Which workflow processes and Robotic platforms are supported?

Identity Automation™ packages are available for workflows using the Promega systems and robotic platforms listed below.

 

Workflow Beckman Coulter Laboratory Automation Workstations Tecan Workstation
Biomek® NXP Biomek® 3000 Freedom EVO®
Differex™ System N/A X N/A
DNA IQ™ System X X X
Plexor® HY System X X X
PCR Setup for
PowerPlex® Systems
Normalization 1 plate 1 plate 1–2 plates
Swab 1–3 plates 1–2 plates 1–4 plates
FTA® Punch 1–5 plates 1–4 plates 1–9 plates
nonFTA Punch 1–5 plates 1–4 plates 1–9 plates

 

How much development time is required to get the robot "working?"

Identity Automation™ solutions work "on delivery." The Promega installer will help with initial settings and provide full training. Subsequent internal validation should be performed by the laboratory. Time for internal validation can vary greatly based on staffing commitment, accreditation guidelines/requirements and the specific process to be automated.

If I automate just one process now, will I be able to add other processes later with the same robot?

If you purchase Identity Automation™ for one process, additional processes can be added to the same platform in the future.

If my Lab's automation expert leaves, how will we retrain our staff?

We offer refresher training services for new staff. Promega has been developing and supporting automation solutions for 10 years. Our proven expertise will be available for years to come.

Will we be forced to change how we set up PCR plates (controls, samples,
spacing, etc.)?

No. The Identity Automation™ normalization and PowerPlex® setup uses the highly flexible STR Normalization Manager™ interface. This interface allows extensive control of laboratory preferences, including plate layout. The flexibility of the Identity Automation™ solutions and interfaces helps fit these methods into a laboratory's current workflow rather than requiring a significant change in workflow to accommodate automation.

Our lab uses a chemistry from another company, will you help automate?

The automation supplier and/or manufacturer of the chemistry are the best resources to provide and support the automation of their chemistry. However, Identity Automation™ solutions are flexible in allowing automated chemistries from other manufacturers to co-exist on the same robotic platforms with Identity Automation™ installations.

Can I use the STR Normalization Manager™ with Quantifiler® or Quantifiler® DUO data?

Yes, the STR Normalization Manager™ will recognize outputs from the Applied Biosystems 7500 SDS software. Of course, it will also accept Plexor® HY data from the Plexor® Analysis Software.

Can I use the STR Normalization Manager™ with nonPowerPlex STR kits?

No. The STR Normalization Manager™ is developed to output directly into robotic normalization and PowerPlex® setup methods.

What throughput can be expected for Identity Automation™ solutions?

Approximate automated liquid handler run times on Beckman Coulter Laboratory Automation Workstations for single-plate setup are shown below. Run times are in minutes and do not include reagent preparation and deck setup. Actual run times may vary depending on sample number, layout, and processing options. Run times with asterisks (*) do not include on-deck preparation of reaction mix.

Workflow Number of Samples Biomek® 3000 Biomek® NXp Biomek® FXp
Differex™ + DNA IQ™ Systems 40 samples 260 N/A N/A
DNA IQ™ System 80 samples 115 100 45
Plexor HY® System 80 samples + standards 35 27 27
Normalization + PCR Set-up 80 samples + controls 125 50 50
Direct Amp Swab PCR Set-up 88 samples + controls 21* / 28 15* / 22 15* / 22
Direct Amp FTA® Punch PCR Set-up 88 samples + controls 11* / 18 6* / 13 6* / 13
Direct Amp nonFTA Punch PCR Set-up 88 samples + controls 13* / 21 7* / 18 7* / 18

Approximate automated liquid handler run times on Tecan Freedom EVO® Workstation with the 4-Tip and 8-Tip configurations for single-plate setup are shown below. Run times are in minutes and do not include reagent preparation and deck setup. Actual run times may vary depending on sample number, layout, and processing options. Run times with asterisks (*) do not include on-deck preparation of reaction mix.

Workflow Number of Samples Freedom EVO®
4-Tip
Freedom EVO®
8-Tip
DNA IQ™ System 80 samples 190 100
Plexor HY® System 80 samples + standards 40 27
Normalization + PCR Set-up 80 samples + controls 93 93
Direct Amp Swab PCR Set-up 88 samples + controls 29* / 36 15* / 22
Direct Amp FTA® Punch PCR Set-up 88 samples + controls 9* / 16 6* / 13
Direct Amp nonFTA Punch PCR Set-up 88 samples + controls 13* / 21 7* / 18