See what everyone is saying about HaloTag^®

• "Purifications using FLAG, 3xFLAG, His(6)Tag and HaloTag were performed in parallel, HaloTag was shown to provide significantly higher yields, purity and overall recovery of the expressed proteins."

  Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr Purif. Apr; 76(2): 154-64.

• "Halo-CARM1 was expressed in HEK293T cells, purified on Halo-link resin, and cleaved with TEV protease to obtain full-length CARM1. Surprisingly, this approach produced a very pure protein…and a significantly higher yield than FLAG-CARM1 purified from Sf9 cells."

  Kuhn, Peter et al. (2011) Automethylation of CARM1 allows coupling of transcription and mRNA splicing. Nucleic Acids Res. Apr; 39(7): 2717–26.

• "This approach was significantly faster and cheaper than a comparable Flag-tag based approach…To the best of our knowledge, the HaloTag-based protein purification methodology is the only one that allows good purification and recovery of target proteins expressed at low levels in either E. coli or mammalian cells."

  Chumanov, R.S., et al. (2011) Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology. Protein Expr. Purif. Apr; 76(2): 145-53.

• "HaloTag enabled us to overcome prior protein expression and purification difficulties. The quick, straightforward protocol let us purify mg-quantities of full-length, enzymatically-active protein from transfected HEK293T cells. It worked great!"

  Dr. Robert Chumanov University of Wisconsin – Madison

• "Starting material size was small but to my surprise it worked and first time in my life I got a purified protein (single band) with a new kit in my first attempt."

  Dr. Fouzia Sadiq Imperial College – London

• "Easy to use, my graduate student got it to work nicely on his first attempt. The purity of the preparation was outstanding."

  Dr. Robert Matts Oklahoma State University

• "This is the simplest system I have ever seen for purification of mammalian recombinant proteins in a state of nearly native form cultured cells."

  Dr. Takahiro Nagase Kazusa DNA Research Institute

• "My laboratory recently published on the use of HaloTag to purify CARM1 from transiently transfected HEK293T cells. This method has proven to be remarkably effective and reproducible. Use of HaloTag has not only allowed us to study this important arginine methyl transferase, but also has become a popular new part of a lab course on protein purification that I teach at Cold Spring Harbor. The students are very impressed by how well it works and can hardly wait to go home and try it on their favorite protein."

  Dr. Richard Burgess Emeritus Professor of Oncology University of Wisconsin – Madison