Luciferase Pathway Reporters

We have developed a number of specific Pathway Reporters and Cell Lines, which are currently available for sale by Quote. Simply mark all those of interest and we will contact you with pricing and delivery time lines.

Note that some materials vary in the selectable marker (e.g., Hygro vs. Puro) or the luc gene portion by a unique PEST sequence (e.g., luc2P vs. luc2CP). Please ensure that you select the desired construct.

Custom materials are developed by the Custom Assay Services team within R&D. Although functionally tested, they are not manufactured under the Promega ISO13485 quality system and are sold “as is” with no warranty either stated or implied.

NanoLuc® Fusion Vectors
Promoter Tag Tag (Location) Vector Name (E. coli selection)
* MCS = Multiple Cloning Site
CMV NanoLuc® N-terminal pFN[Nluc/CMV/Neo] Vector (Amp)
CMV NanoLuc® N-terminal pFN[Nluc/CMV/Neo] Vector (Kan)
CMV NanoLuc® C-terminal pFC[Nluc/CMV/Neo] Vector (Amp)
CMV NanoLuc® C-terminal pFC[Nluc/CMV/Neo] Vector (Kan)
CMV secreted NanoLuc® N-terminal pFN[secNluc/CMV/Neo] Vector (Amp)
CMV secreted NanoLuc® N-terminal pFN[secNluc/CMV/Neo] Vector (Kan)
CMV secreted NanoLuc® C-terminal pFC[secNluc/CMV/Neo] Vector (Amp)
CMV secreted NanoLuc® C-terminal pFC[secNluc/CMV/Neo] Vector (Kan)
CMVd2 (weakened CMV) NanoLuc® N-terminal pFN[Nluc/CMVd2/Neo] Vector (Kan)
CMVd2 (weakened CMV) NanoLuc® C-terminal pFC[Nluc/CMVd2/Neo] Vector (Kan)
CMVd3 (weakened CMV) NanoLuc® N-terminal pFN[Nluc/CMVd3/Neo] Vector (Kan)
CMVd3 (weakened CMV) NanoLuc® C-terminal pFC[Nluc/CMVd3/Neo] Vector (Kan)
CMV NanoLuc® N-terminal pN[Nluc/MCS/CMV/Neo] Vector (Kan)*
CMV NanoLuc® C-terminal pC[Nluc/MCS/CMV/Neo] Vector (Kan)*
CMV Secreted NanoLuc® N-terminal pN[secNluc/MCS/CMV/Neo] Vector (Kan)*
CMV NanoLuc® Control pN[Nluc/CMV/Neo] Vector (Kan)

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NanoLuc®-HaloTag® Fusion Reporter
Promoter Reporter Vector Name (E. coli selection)
PGK NanoLuc®-HaloTag® Fusion p[Nluc-HT/PGK/Neo] Vector
PGK NanoLuc®-HaloTag®-PEST Fusion p[Nluc-HT-PEST/PGK/Neo] Vector

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NanoLuc-Protein Fusion Reporter
Promoter Reporter Vector Name (E. coli selection)
CMV NRF2- NanoLuc® Fusion p[NFE2-Nluc/CMV/Neo] Vector
CMV p53- NanoLuc® Fusion p[p53-Nluc/CMV/Neo] Vector
CMV HIF1a- NanoLuc® Fusion p[HIF1a-Nluc/CMV/Neo] Vector

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Signal Pathways Using NanoLuc as Reporter Gene
Pathway Response Element/Promoter Vector Name
cAMP/PKA Cyclic AMP Response Element (CRE) pNL[NlucP/CRE/Hygro] Vector
Ras/MEK-1 Serum Response Element (SRE) pNL[NlucP/SRE/Hygro] Vector
RhoA (Gα12/13) Serum Response Factor Response Element (SRF) pNL[NlucP/SRF/Hygro] Vector
Calcium/Calcineurin Nuclear Factor of Activated T-Cells (NFAT) Response Element pNL[NlucP/NFAT-RE/Hygro] Vector
Oxidative Stress Antioxidant Response Element (ARE) pNL[NlucP/ARE/Hygro] Vector
DNA Damage p53 response element (p53 RE) pNL[NLucP/p53-RE/Hygro] Vector
ER Stress Activating Transcription Factor 4 response element (ATF4 ERSE) pNL[NlucP/ATF4-RE/Hygro] Vector
ER Stress Activating Transcription Factor 6 response element (ATF6 ERSE) pNL[NlucP/ATF6-RE/Hygro] Vector
Heavy Metal Stress Metal Regulatory Element (MRE) pNL[NlucP/MRE/Hygro] Vector
Hypoxia Hypoxia Response Element (HRE) pNL[NlucP/HRE/Hygro] Vector
Xenobiotic Stress Xenobiotic Responsive Element (XRE) pNL[NlucP/XRE/Hygro] Vector
MAPK/JNK AP1 Response Element (AP1 RE) pNL[NlucP/AP1-RE/Hygro) Vector
PI3K/Akt/MAPK Myc/Max Response Element pNL[NlucP/MycMax-RE/Hygro] Vector
JAK/STAT5 IL3 STAT5 Response Element pNL[NlucP/STAT5-RE/Hygro] Vector
TGF-β SMAD3/SMAD4 Binding Element (SBE) pNL[NlucP/SBE/Hygro] Vector
JAK/STAT3 IL-6 Sis-inducible Element (SIE) Response Element pNL[NlucP/SIE/Hygro] Vector
JAK/STAT1/2 IFN-α Interferon Stimulated Response Element (ISRE) pNL[NlucP/ISRE/Hygro]d Vector
Wnt TCF-LEF Response Element (TCF-LEF RE) pNL[NlucP/TCF/LEF-RE/Hygro]d Vector
JAK/STAT1 IFN-γ IFN-γ activation site (GAS) Response Element pNL[NlucP/GAS-RE/Hygro]d Vector
Multiple CEBP Response Element pNL[NlucP/CEBP-RE/Hygro] Vector
Control minP pNL[NLucP/minP/Hygro] Vector
Control CMV pNL[NlucP/CMV/Hygro] Vector
Control CMVd3 pNL[NlucP/CMVd3/Neo] Vector

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NanoLuc Cell Lines
Pathway Reporter Cell Line Background
NFkB pNL[NlucP/NFkB-RE/Hygro] Vector HEK293
Control pNL[NlucP/CMVd3/Neo] Vector HEK293

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Firefly Luciferase Cell Lines
Pathway Reporter Cell Line Background
Control pGL4[luc2/CMV/Hygro] Vector HEK293
Control pGL4[luc2P/CMVd3/Hygro] Vector HEK293
JAK/STAT1 IFN-γ pGL4[luc2P/GAS-RE/Hygro] Vector HepG2
JAK/STAT1 IFN-γ pGL4[luc2P/GAS-RE/Hygro] Vector HeLa
JAK/STAT3 IL6 pGL4.47[luc2P/SIE-RE/Hygro] Vector HEK293
JAK/STAT3 IL6 pGL4.47[luc2P/SIE-RE/Hygro] Vector HepG2
Antioxidant pGL4.37[luc2P/ARE/Hygro] Vector HEK293
Hypoxia pGL4.42[luc2P/HRE/Hygro] Vector HEK293
Hypoxia pGL4.42[luc2P/HRE/Hygro] Vector HepG2
MAPK pGL4.44[luc2P/AP1 RE/Hygro] Vector HEK293
Ras/MEK-1 pGL4.33[luc2P/SRE/Hygro] Vector HEK293
RhoA (Gα12/13) pGL4.34[luc2P/SRF-RE/Hygro] Vector HEK293
Calcium/Calcineurin pGL4[luc2P/NFAT-RE/Hygro] Vector Jurkat

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pGL4 Signaling Vectors
Pathway Response Element/Promoter Vector Name
Hedgehog Gli Response Element (Gli) pGL4[luc2P/Gli-RE/Hygro] Vector
JAK/STAT1 IFN-γ IFN-γ activation site (GAS) Response Element pGL4[luc2P/GAS-RE/Hygro] Vector
Multiple C/EBP Response Element (C/EBP) pGL4[luc2P/C/EBP-RE/Hygro] Vector
Wnt TCF-LEF Response Element (TCF-LEF RE) pGL4[luc2CP/TCF-LEF RE/Hygro] Vector
DNA damage/p53 p53 Response Element (p53-RE) pGL4[hRlucP/p53-RE/Hygro] Vector
DNA damage/p53 p53 Response Element (p53-RE) pGL4[luc2P/p53-RE/Puro] Vector
ER Stress Activating Transcription Factor 4 Response Element (ATF4-RE) pGL4[luc2P/ATF4-RE/Hygro] Vector
Hypoxia Hypoxia Response Element (HRE) pGL4[hRlucP/HRE/Hygro] Vector
JAK/STAT4 IL12 IRF1 Response Element pGL4[luc2P/STAT4-RE/Hygro] Vector
MAPK AP1 Response Element (AP1 RE) pGL4[hRlucP/AP1 RE/Hygro] Vector
Myc Myc Response Element (Myc) pGL4[luc2P/Myc/Hygro] Vector
Notch CBF-1/RBP-JK Response Element pGL4[luc2P/RBP-JK-RE/Hygro] Vector
IL1 Human IL8 promoter pGL4[luc2P/hIL8/Hygro] Vector
T cell activation, IL1 Human IL2 promoter pGL4[luc2P/IL-2/Hygro] Vector
T cell activation, IL1 Human IL2 promoter pGL4[luc2P/IL-2/Puro] Vector
NGF Human Early Growth Response factor-1(EGR 1) Promoter pGL4[luc2P/hEGR1/Hygro] Vector
NFAT NFAT Response Element pGL4[luc2P/NFAT-RE/Puro] Vector
TGF-β/BMP Human DNA-binding protein inhibitor ID-1 Promoter pGL4[luc2P/hID1/Hygro] Vector
Androgen activation in prostate cancer Human PSA promoter pGL4[luc2P/PSA-long/Hygro] Vector
STAT3 Human G-CSF pGL4[luc2P/GCSF/Hygro] Vector
Cytochrome P450/drug metabolism Human 3A4 pGL4[luc2P/3A4/Hygro] Vector
Cytochrome P450/drug metabolism Human 2B6 pGL4[luc2P/2B6/Hygro] Vector
Control CMVd3 pGL4 [luc2P/CMVd3/Hygro] Vector
Control CMV promoter pGL4[hRlucP/CMV/Hygro] Vector
Control CMV promoter pGL4[luc2P/CMV/Hygro] Vector
Control Minimal promoter (minP) pGL4[hRlucP/minP/Hygro] Vector

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RLuc-Protein Fusion Reporter
Promoter Reporter Vector Name (E. coli selection)
CMV Rluc-ODD Fusion p[Rluc-ODD/CMV/Neo] Vector
SV40 Rluc-Neo Fusion p[Rluc-Neo/SV40] Vector
CMV Rluc Control pGL4[hRluc/CMV/Hygor] Vector

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Can't find what you're looking for?

Some custom vectors may now be available as Promega products. Check this list for more information.

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