Luciferase Pathway Reporters

We have developed a number of specific Pathway Reporters and Cell Lines, which are currently available for sale by Quote. Simply mark all those of interest and we will contact you with pricing and delivery time lines.

Note that some materials vary in the selectable marker (e.g., Hygro vs. Puro) or the luc gene portion by a unique PEST sequence (e.g., luc2P vs. luc2CP). Please ensure that you select the desired construct.

Custom materials are developed by the Custom Assay Services team within R&D. Although functionally tested, they are not manufactured under the Promega ISO13485 quality system and are sold “as is” with no warranty either stated or implied.

Luciferase Pathway Reporters

NanoLuc® Fusion Vectors
  Promoter Tag Tag (Location) Vector Name
CMV NanoLuc® N-terminal pFN[Nluc/CMV/Neo] Vector (Amp)
CMV NanoLuc® N-terminal pFN[Nluc/CMV/Neo] Vector (Kan)
CMV NanoLuc® C-terminal pFC[Nluc/CMV/Neo] Vector (Amp)
CMV NanoLuc® C-Terminal pFC[Nluc/CMV/Neo] Vector (Kan)
CMV Secreted NanoLuc® N-terminal pFN[secNluc/CMV/Neo] Vector (Amp)
CMV Secreted NanoLuc® N-terminal pFN[secNluc/CMV/Neo] Vector (Kan)
CMV Secreted NanoLuc® C-terminal pFC[secNluc/CMV/Neo] Vector (Amp)
CMV Secreted NanoLuc® C-terminal pFC[secNluc/CMV/Neo] Vector (Kan)
CMVd2 (weakened CMV) NanoLuc® N-terminal pfN[Nluc/CMVd2/Neo] Vector (Kan)
CMVd2 (weakened CMV) NanoLuc® C-terminal pFC[Nluc/CMVd2/Neo] Vector (Kan)
CMVd3 (weakened CMV) NanoLuc® N-terminal pFN[Nluc/CMVd3/Neo] Vector (Kan)
CMVd3 (weakened CMV) NanoLuc® C-terminal pFC[Nluc/CMVd3/Neo] Vector (Kan)
CMV NanoLuc® N-terminal pN[Nluc/MCS/CMV/Neo] Vector (Kan)*
CMV NanoLuc® C-terminal pC[Nluc/MCS/CMV/Neo] Vector (Kan)*
CMV Secreted NanoLuc® N-Terminal pN[secNluc/MCS/CMV/Neo] Vector (Kan)*
CMV NanoLuc® Control pN[Nluc/CMV/Neo] Vector (Kan)
CMVd3 NanoLuc® Control pNL[NlucP/CMVd3/Neo] Vector

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NanoLuc®-HaloTag® Fusion Reporters
  Promoter Reporter Vector Name (E. coli selection)
PGK NanoLuc® HaloTag® Fusion p[Nluc-HT/PGK/Neo] Vector
PGK NanoLuc® HaloTag® PEST Fusion p[Nluc-HT-PEST/PGK/Neo] Vector

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NanoLuc®-Protein Fusion Reporter
  Promoter Reporter Vector Name (E. coli selection)
CMV p53NanoLuc® Fusion p[p53-Nluc/CMV/Neo] Vector

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Luciferase Reporter Vectors for Signal Pathways
  Pathway Response Element/Promoter Vector Name
cAMP/PKA Cyclic Amp Response Element (CRE) pNL[NlucP/CRE/Hygro] Vector
Ras/MEK1 Serum Response Element pNL[NlucP/SRE/Hygro] Vector
RhoA (Gα12/13) Serum Response Factor Response Elemnet (SRF) pNL[NlucP/SRF/Hygro] Vector
RhoA (Gα12/13) Serum Response Factor Response Element (SRF) pGL4(Luc2P/SRF-RE/Puro) Vector
Calcium/Calcineurin Nuclear Factor of Activated TCells (NFAT) Response Element pNL[NlucP/NFAT-RE/Hygro] Vector
Calcium/Calcineurin Nuclear Factor of Activated T-Cells (NFAT) Response Element pGL4(Luc2P/NFAT-RE/Hygro) Vector
Oxidative Stress Antioxidant Response Element (ARE) pNL[NlucP/ARE/Hygro] Vector
DNA Damage p53 Response Element (p53 RE) pNL[NLucP/p53-RE/Hygro] Vector
DNA damage/p53 p53 Response Element (p53RE) pGL4[hRlucP/p53-RE/Hygro] Vector
DNA damage/p53 p53 Response Element (p53RE) pGL4[luc2P/p53-RE/Puro] Vector
ER Stress Activating Transcription Factor 4 Response Element (ATF4 ERSE) pNL[NlucP/ATF4-RE/Hygro] Vector
ER Stress Activating Transcription Factor 6 Response Element (ATF6 ERSE) pNL[NlucP/ATF6-RE/Hygro] Vector
ER Stress Activating Transcription Factor 4 Response Element (ATF4RE) pGL4[luc2P/ATF4-RE/Hygro] Vector
Heavy Metal Stress Metal Regulatory Element (MRE) pNL[NlucP/MRE/Hygro] Vector
Hypoxia Hypoxia Response Element (HRE) pNL[NlucP/HRE/Hygro] Vector
Hypoxia Hypoxia Resposne Element (HRE) pGL4[hRlucP/HRE/Hygro] Vector
Xenobiotic Stress Xenobiotic Responsive Element (XRE) pNL[NlucP/XRE/Hygro] Vector
MAPK/JNK AP1 Response Element (AP1 RE) pNL[NlucP/AP1-RE/Hygro] Vector
MAPK/JNK AP1 Response Element (AP1 RE) pGL4[hRlucP/AP1-RE/Hygro] Vector
MAPK/JNK AP1 Response Element (AP1 RE) pGL4[luc2P/AP1-RE/Hygro] Vector
PI3K/Akt/MAPK Myc/Max Response Element pNL[NlucP/MycMax-RE/Hygro] Vector
TGFβ SMAD3/SMAD4 Binding Element (SBE) pNL[NlucP/SBE/Hygro] Vector
TGFβ/BMP Human DNA-binding Portein Inhibitor ID-1 Promoter pGL4[luc2P/hlD1/Hygro] Vector
JAK/STAT5 IL3 STAT5 Response Element pNL[NlucP/STAT5-RE/Hygro] Vector
JAK/Stat3 IL6 Sis-inducible Element (SIE) Response Element pNL[NlucP/SIE/Hygro] Vector
JAK/STAT1/2 IFNα Interferon Stimulated Response Element (ISRE) pNL[NlucP/ISRE/Hygro] Vector
JAK/STAT1 IFNγ IFNγ Activation Site (GAS) Response Element pNL[NlucP/GAS-RE/Hygro] Vector
JAK/STAT4 IL12 IRF1 Response Element pGL4[luc2P/STAT4-RE/Hygro] Vector
JAK/STAT1 IFNγ IFNγ Activation Site (GAS) Response Element pGL4[luc2P/GAS-RE/Hygro] Vector
Wnt TCF-LEF Response Element (TCFLEF RE) pNL[NlucP/TCF/LEF-RE/Hygro] Vector
Wnt TCF-LEF Response Element (TCF-LEF RE) pGL4[luc2CP/TCF-LEF RE/Hygro] Vector
Multiple C/EBP Response Element pNL[NlucP/C/EBP-RE/Hygro] Vector
Multiple C/EBP Response Element (C/EBP) pGL4[luc2P/C/EBP-RE/Hygro] Vector
Hedgehog Gli Response Element (Gli) pGL4[luc2P/Gli-RE/Hygro] Vector
Myc Myc Response Element (Myc) pGL4[luc2P/Myc/Hygro] Vector
Notch CBF1/RBPJk Response Element pGL4[luc2P/RBP-Jk-RE/Hygro] Vector
IL17 Human LCN2 (Lipocalin) Promoter pGL4[luc2P/LCN2/Hygro] Vector
IL1 Human IL8 Promoter pGL4[luc2P/hIL8/Hygro] Vector
T Cell Activation, IL1 Human IL2 Promoter pGL4[luc2P/IL-2/Hygro] Vector
T Cell Activation, IL1 Human IL2 Promoter pGL4[luc2P/IL-2/Puro] Vector
NGF Human Early Growth Response Factor1 (EGR 1) Promoter pGL4[luc2P/hEGR1/Hygro] Vector
NFAT NFAT Response Element pGL4[luc2P/NFAT-RE/Puro] Vector
Androgen Activation in Prostate Cancer Human PSA Promoter pGL4[luc2P/PSA-long/Hygro] Vector
STAT3 Human G-CSF pGL4[luc2P/GCSF/Hygro] Vector
Cytochrome 450/Drug Metabolism Human 3A4 pGL4[luc2P/3A4/Hygro] Vector
Cytochrome 450/Drug Metabolism Human 2B6 pGL4[luc2P/2B6/Hygro] Vector
VEGF2 hVEGFR2 Promoter pGL4(Luc2P/hVEGFR2(-290 to +269)/Hygro) Vector
Control minP pNL[NLucP/minP/Hygro] Vector
Control CMV pNL[NlucP/CMV/Hygro] Vector
Control CMVd3 pNL[NlucP/CMVd3/Neo] Vector
Control CMVd3 pGL4[luc2P/CMVd3/Neo] Vector
Control CMV Promoter pGL4[hRlucP/CMV/Hygro] Vector
Control CMV Promoter pGL4[luc2P/CMV/Hygro] Vector
Control Minimal Promoter (minP) pGL4[hRlucP/minP/Hygro] Vector

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NanoLuc® Cell Lines
  Pathway Description Response Element
NFkB pNL[NlucP/NFkBRE/Hygro] Vector HEK293
Control pNL[NlucP/CMVd3/Neo] Cell Line HEK293

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Cell Lines
  Pathway Description Response Element
Use for TNFa Blocker Assay Thaw-and-use U937 Cells No Luciferase Reporter in these cells
GPCR GloSensor-22F cAMP/Aequorin HEK293 Cell Line 22F, cAMP + Aequorin
Calcium/Calcineurin T Cell Activation GloResponse™ NFAT-luc2 Jurkat Cell Line Nuclear Factor of Activated T-Cells (NFAT) Response Element
Calcium/Calcineurin T Cell Activation Thaw and Use GloResponse™ NFAT-luc2 Jurkat Cells – 1X Nuclear Factor of Activated T-Cells (NFAT) Response Element
Calcium/Calcineurin T Cell Activation Thaw and Use GloResponse™ NFAT-luc2 Jurkat Cells – 5X Nuclear Factor of Activated T-Cells (NFAT) Response Element
Calcium/Calcineurin T Cell Activation GloResponse™ NFAT-luc2P Jurkat Cell Line Nuclear Factor of Activated T-Cells (NFAT) Response Element
NFkB Thaw and Use GloResponse NF-kB-RE-luc2P HEK293 Cells Nuclear Factor kB Response Element
Murine FcγRIV ADCC Murine FcγRIV ADCC Reporter Bioassay, Core Kit Nuclear Factor of Actiavted T-Cells (NFAT) Response Element
Murine FcγRIV ADCC Murine FcγRIV ADCC Reporter Bioassay, Complete Kit Nuclear Factor of Actiavted T-Cells (NFAT) Response Element
Murine FcγRIV ADCC Murine FcγRIV ADCC Bioassay Effector Cells (CPM) Nuclear Factor of Activated T-Cells (NFAT) Response Element
FcγRIIa H Variant ADCP GloResponse™ NFAT-luc2/FcgRIIa (H131 variant) Jurkat Cell Line Nuclear Factor of Activated T-Cells (NFAT) Response Element
FcγRlla H Variant ADCP FcγRlla H variant ADCP Bioassay Core Kit Nuclear Factor of Activated T-Cells (NFAT) Response Element
FcγRlla H Variant ADCP FcγRlla H variant ADCP Bioassay Complete Kit Nuclear Factor of Activated T-Cells (NFAT) Response Element
Oxidative Stress GloResponse ARE-luc2P HEK293 Cell Line Antioxidant ARE
Hypoxia GloResponse HRE-luc2P HepG2 Cell Line Hypoxia Response Element (HRE)
EGF Responsive GloResponse SRE-luc2P HEK293 Cell Line Serum SRE
Calcium/Calcimeurin GloResponse NFAT-luc2P/KDR HEK293 Cell Line NFAT + KDR
GPCR GloSensor-40F cGMP HEK293 Cell Line 40F, cGMP
GPCR GloSensor-42F cGMP HEK293 Cell Line 42F, cGMP
GITR Activation BioAssay GloResponse NFkB-luc2/GITR Jurkat cells IL2 promoter + CTLA-4
mTNFa mTNFα CHO-K1 Target Cell Line TNFα mutant
mTNFa Thaw-and-Use mTNFα CHO-K1 Cells TNFα mutant
RhoA (Gα12/13) GloResponse SRF-luc2P HEK293 Cell Line Serum Response Factor SRF
IL6 responsive; use for Ab potency assay GloResponse SIE-luc2P HepG2 Cell Line Sis inducible element SIE
CTLA4 Blockade Bioassay CTLA-4 Blockade Assay System, Propagation Model IL2 promoter
CTLA4 Blockade Bioassay Thaw-and-Use CTLA4 Blockade Assay IL2 promoter
CTLA4 Blockade Bioassay Thaw-and-Use CTLA4 Blockade Assay, 5X IL2 promoter
T cell activation GloResponse IL2-luc2P Jurkat Cell Line IL2 promoter
T cell activation Thaw and Use, GloResponse™ IL2-luc2P Jurkat Cells IL2 promoter
T cell activation Thaw and Use, GloResponse™ IL2-luc2P Jurkat Cells, 5X IL2 promoter
Thaw-and-use PD-L1 negative Cells Thaw-and-use PD-L1 negative Cells No Luciferase Reporter in these cells
PD-1/PD-L1 Blockade Assay PD1/PD-L1 Blockade Assay NFAT + PD-1
PD-1/PD-L1 Blockade Assay PD1/PD-L1 Blockade Assay, 5X NFAT + PD-1
PD-1/PD-L1 Blockade Assay PD1/PD-L1 Blockade Assay System, Prop NFAT + PD-1
T cell activation Human T-Activator Cells, Propagation Model No Luciferase Reporter in these cells
IL6 responsive; use for Ab potency assay GloResponse SIE-luc2P HEK293 Cell Line Sis inducible element SIE
NFkB GloResponse NFkB-NlucP HEK293 Cell Line Nuclear Factor kB Response Element
IFNβ & IFNα responsive GloResponse ISRE-luc2P HEK293 Cell Line Interferon Stimulated ISRE
IL6 responsive; use for Ab potency assay Thaw and Use GloResponse™ SIE-Luc2P/HEK293 Cells Sis inducible element SIE
CHOK1 GloSensor-23F cAMP/Rat Y2 CHO Cell Line 23F, cAMP, + Rat Y2 receptor
CD40 Activation Bioassay CD40 Bioassay, Thaw and Use Cells, Ramos AP1/NFkB
LAG3 LAG3 Effector Cell Line Nuclear Factor of Activated T-Cells (NFAT) Response Element
Immune Stimulatory 4-1BB Thaw-and-Use-GloResponse NfkB-luc2/4-1BB Jurkat Cells Nuclear Factor kB Response Element
Immune Stimulatory OX4D Thaw-and-Use-GloResponse NFkB-luc2/OX40 Jurkat Cells Nuclear Factor of Activated T-Cells (NFAT) Response Element
GloSensor-20F cAMP HEK293 Cell Line 20F, cAMP
cAMP/PKA GloResponse CRE-luc2P HEK293 Cell line Cyclic AMP Response Element (CRE)
Calcium/Calcineurin GloResponse NFAT-luc2P HEK293 Cell line Nuclear Factor of Activated T-Cells (NFAT) Response Element
GloResponse NFkB-luc2P HEK293 Cell line NFkB
GloResponse 9XGAL4 UAS-luc2P HEK293 Cell line 9XGAL4 UAS
MAPK/JNK GloResponse AP1-luc2P HEK293 Cell Line AP1 Response Element (AP1 RE)
Estrogen responsive GloResponse ERα-UAS-luc2P HEK293 Cell Line Estrogen Receptor ERα-UAS
IFNγ responsive; use for Ab potency assay GloResponse GAS-luc2P HeLa Cell Line IFN-γ Activation site GAS
IFNγ responsive; use for Ab potency assay GloResponse GAS-luc2P HepG2 Cell Line IFN-γ Activation site GAS
Responds to NGFβ GloResponse hEGR-luc2P PC12 Cell Line hEGR promoter
Hypoxia GloResponse HRE-luc2P HEK293 Cell Line Hypoxia Response Element (HRE)
IL-1 responsive GloResponse IL8-luc2P A549 Cell Line IL8 promoter
EPO responsive GloResponse STAT5-luc2P TF1 Cell Line STAT5
GPCR DRD1 GloSensor-20F cAMP/DRD1 HEK293 Cell Line 20F, cAMP, + DRD1 receptor
GPCR DRD2 GloSensor-20F cAMP/DRD2 HEK293 Cell Line 20F, cAMP, + DRD2 receptor
GPCR GloSensor-22F cAMP HEK293 Cell Line 22F, cAMP
GPCR MC4R GloSensor-22F cAMP/MC4R HEK293 Cell Line 22F, cAMP, + MC4R receptor
GPCR GloSensor-23F cAMP CHO-K1 Cell Line 23F, cAMP
Apoptosis, in vivo imaging GloSensor-30F DEVD Caspase DLD1 Cell Line 30F, Caspase 3/7
Apoptosis, in vivo imaging GloSensor-30F DEVD Caspase MDA-MB231 Cell Line 30F, Caspase 3/7
GPCR GloSensor-L9 cAMP HEK293 Cell Line L9, cAMP
Control pGL4[luc2/CMV/Hygro] Cell Line HEK293
Control pGL4[luc2P/CMVd3/Neo] Cell Line HEK293

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Renilla Luc Protein Fusion Reporter
  Promoter Reporter Vector Name (E. coli system)
CMV RlucODD Fusion p[RlucODD/CMV/Neo] Vector
SV40 RlucNeo Fusion p[RlucNeo/SV40] Vector
CMV Rluc Control pGL4[hRluc/CMV/Hygro] Vector

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HaloTag Vectors

CMV Promoter
  Promoter Tag (Location) Vector Name (E. coli selection)
CMVd1 HaloTag® (C-terminal) pFC206k HaloTag® CMVd1neoFlexi® Vector (Kan)
CMVd2 HaloTag® (C-terminal) pFC207K HaloTag® CMVd2neoFlexi® Vector (Kan)
CMVd1 HaloTag® (N-terminal) pFN208K HaloTag® CMVd1neoFlexi® Vector (Kan)
CMVd2 HaloTag® (N-terminal) pFN209K HaloTag® CMVd2neoFlexi® Vector (Kan)
CMV SS*HaloTag® (N-terminal) pFN210A SSHaloTag® CMVneoFlexi® Vector (Amp)
CMVd1 HaloTag® NLS** (C-terminal) pFC211A HaloTag® NLS CMVd1 Flexi® Vector (Amp)
CMVd2 HaloTag® NLS** (C-terminal) pFC212A HaloTag® NLS CMVd2 Flexi® Vector (Amp)

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Other Mammalian Promoters
  Promoter Tag (location) Vector Name (E. coli selection)
Regulated Mammalian HaloTag® (C-terminal) pFC200A HaloTag® RM Flexi® Vector (Amp)
Thymidine Kinase HaloTag® (C-terminal) pFC201A HaloTag® TK Flexi® Vector (Amp)
SV40 HaloTag® (C-terminal) pFC202A HaloTag® SV40 Flexi® Vector (Amp)
PGK HaloTag® (N-terminal) pFC203A HaloTag® PGKneo Flexi® Vector (Amp)
PGK HaloTag® (C-terminal) pFC204A HaloTag® PGKneo Flexi® Vector (Amp)
EF1α HaloTag® (N-terminal) pFN205K HaloTag® EF1alphaneo Flexi® Vector (Kan)

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Plasmid Constructs for GPCR/Second Messenger Studies
  Second Messenger Construct Variant Vector Name
cAMP 21F pGloSensor™ 21F cAMP
cAMP 23F pGloSensor™ 23F cAMP
cGMP 40F pGloSensor™ 40F cGMP
cGMP 42F pGloSensor™ 42F cGMP

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Protease Glo-Sensors
  Second Messenger Construct Variant Vector Name
Caspase DEVD 30F pGloSensor™ -30DEVDG Caspase
Granzyme N/A pGloSensor™ - IETD
  Protease GloSensor Stable Cell Lines Recommended Applications Cell Background
GloSensor 30F, Caspase 3/7 GloSensor-30F DEVD Caspase DLD1 Cell Line DLD1 Cell Line
GloSensor 30F, Caspase 3/7 GloSensor-30F DEVD Caspase MDA-MB231 Cell Line MDA-MB231 Cell Line

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Cell Lines for GPCR/Second Messenger Studies
  cAMP Stable Cell Lines Description Cell Background
GloSensor™20F cAMP HEK293DRD1 Double stable expressing DRD1 receptor HEK293
GloSensor™20F cAMP HEK293DRD2 Double stable expressing DRD2 receptor HEK293
GloSensor™22F cAMP HEK293 Maximum dynamic range in HEK293 Cell Background HEK293
GloSensor™23F cAMP CHOK1 Optimized biosensor for CHO cell background HEK293
GloSensor™22F cAMP/Aequorin HEK293 Multiplexed readout for cAMP and Ca2+ pathways HEK293
MC4R GloSensor™22F cAMP HEK293 Double stable expressing MC4 receptor HEK293
Rat Y2 GloSensor™23F cAMP CHO Double stable expressing rat Y2 receptor (Gicoupled) CHO
GloSensor L9, cAMP GloSensor-L9 cAMP HEK293 cell line
  cGMP Stable Cell Lines Recommended Applications Cell Background
GloSensor™40F cGMP HEK293 pGloSensor™40F cGMP with 2.5µM EC in vitro HEK293
GloSensor™42F cGMP HEK293 pGloSensor™42F cGMP with 0.5µM EC in vitro HEK293

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Can't find what you're looking for?

Some custom vectors may now be available as Promega products. Check this list for more information.

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