|
|
The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "Bioluminescence:
one technology - many faces"
 This
is a Basic webinar,
these webinars address the basics of
specific topics and technologies, such as genetic
reporters or apoptosis. They are especially useful for
students, technicians and junior scientists in general.
Information
Bioluminescence: one technology - many
faces
Introduction to the many applications of bioluminescent assays
The complexity of the cellular environment complicates the analysis of
biochemical changes occurring inside of cells. Bioluminescence provides a
unique technology for accurately quantifying physiological parameters within
the cell. Over the years, we have exploited the chemistry of the
bioluminescent reaction to assay reporter gene activity, changes in ATP
levels, and the enzymatic conversion of pro-luciferin substrates. By
allowing one of the reaction components to vary while keeping the others
fixed, these assays can be used to sensitively quantify promoter elements,
cell surface and nuclear receptor activation, viable cell number or
enzymatic activities. All of the assay results benefit from the high
sensitivity, low background and large dynamic range of bioluminescent
technology as compared to fluorescence. Recently, a novel genetically
engineered firefly luciferase was developed as biosensor. This biosensor can
detect specific molecular interactions through real-time modulation of its
luminescence activity. Applications of this type of biosensor are: real-time
monitoring of cAMP in live cells, detection of protease activity and
specificity, etc. All these strategies to exploit bioluminescence have led
to the formation of a comprehensive portfolio of integrated bioluminescent
products including cell-based assay reagents, hardware, software and support
to help researchers successfully explore gene, protein and cellular
interactions.
This webinar was
presented in the past so registration is not possible
.
|
|