The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
We would like to invite you for the free
webinar, which will be held on
14, 2011. The title is: "Highly
Efficient Purification of Functional Proteins
from Mammalian Cells Using HaloTag".
This is a
specialist webinar. Specialist webinars address
more specialized topics and are useful for PhD
students and senior scientists.
Although mammalian cells are preferred for producing functional mammalian
proteins with appropriate post-translational modifications, purification of
recombinant proteins is typically hampered by low expression levels. We
have addressed this by creating a new method configured for mammalian cell
culture, providing both rapid detection and efficient purification of
mammalian proteins from their native environment. This approach is based on
a protein fusion tag, HaloTag, which provides efficient protein purification
through covalent immobilization coupled with proteolytic tag removal.
HaloTag capabilities would be demonstrated through case studies of purifying
multiple functional kinases from mammalian cells.
is presented by
Rachel Friedman Ohana, Senior R&D scientist at