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Promega @cademy webinar: "Highly Efficient Purification of Functional Proteins from Mammalian Cells Using HaloTag"
 

 

The Promega @cademy provides a tailored programme of non-commercial live webinars, covering a wide range of scientific topics, from very basic concepts to highly technical research presentations. We would like to invite you for the free webinar, which will be held on Tuesday June 14, 2011. The title is: "Highly Efficient Purification of Functional Proteins from Mammalian Cells Using HaloTag".

 

Specialist Webinars


This is a specialist webinar. Specialist webinars address more specialized topics and are useful for PhD students and senior scientists.
 


 

Information

Although mammalian cells are preferred for producing functional mammalian proteins with appropriate post-translational modifications, purification of recombinant proteins is typically hampered by low expression levels.  We have addressed this by creating a new method configured for mammalian cell culture, providing both rapid detection and efficient purification of mammalian proteins from their native environment.  This approach is based on a protein fusion tag, HaloTag, which provides efficient protein purification through covalent immobilization coupled with proteolytic tag removal. HaloTag capabilities would be demonstrated through case studies of purifying multiple functional kinases from mammalian cells.


Rachel Friedman OhanaThis webinar is presented by Rachel Friedman Ohana, Senior R&D scientist at Promega Corporation.


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