The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
The title of this webinar is: "Detection and
Applications of Cell-Free expressed proteins".
This is a basic webinar. Basic webinars address
the basics of specific topics and technologies.
They are especially useful for students,
technicians and junior scientists in general.
Cell-free expression is a convenient
method for rapidly producing protein. Coupled transcription-translation
systems provide the advantage of using plasmid DNA as the temple for
cell-free expression with no need to first transcript the DNA. The
researcher not only has flexibility in which template to use, but also in
which system to use as there are now mammalian, plant, bacterial and insect
cells systems. Protein detection is a requirement for most applications.
Traditionally, one would add radioactive [35S]-methionine to their cell-free
expression reactions. With increased regulations on radioactivity,
researchers are now using other detection methods such as Fluorotech,
Transcend, and Western blotting. Researchers are using cell-free expressed
proteins in many applications including protein:protein pulldowns,
protein:nucleuic acid shift assays, and enzymatic assays. With new systems
that can produce large amounts of protein, researchers are expanding this
list of applications.
was presented by Brad Hook PhD, Sr Application Scientist, Promega
This webinar was
presented in the past so registration is not possible