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The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "Improving
Protein Identification and Characterization
through more efficient protease digestion".
This is a
Specialist webinar,
these webinars addresses more specialized
topics and are useful for PhD students and senior
scientists.
Information
Mass spectrometry has become a standard
method for the identification, quantification and characterization of
proteins present over a wide dynamic range in complex biological matrices.
To this end in practice proteins are most often digested into peptides,
measured by mass spectrometry and matched to known sequences through
algorithm-based searching. While instrumentation and bioinformatics
ultimately determine the depth of complexity in which a sample can be
measured, efficient digestion and high quality proteases are necessary to
produce an experimental data set that will maximize the potential for
correct identification and characterization. In this presentation we will
discuss several parameters from the biological side of protein analysis to
help get the most out of protein identification and characterization
experiments. The focus will be on the complimentary use of proteases and
conditions for their use.
 This webinar
was presented by Dan Simpson PhD, R&D Biochemical & Cellular
Technologies, Promega Corporation, USA
This webinar was
presented in the past so registration is not possible
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