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The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "Monitoring Human
Stem Cell Differentiation and Pluripotency via
Multiplex qPCR".
This is a
Specialist webinar,
these webinars addresses more specialized
topics and are useful for PhD students and senior
scientists.
Information
Advances in gene
expression technologies have led to the identification of molecular markers
associated with stem cell pluripotency and differentiation. Quantitative PCR,
in particular, has proven to be a valuable method in nucleic acid detection;
by measuring PCR product accumulation in real time, this technique provides
greater sensitivity and more accurate quantitation than traditional endpoint
PCR. Coupled with reverse transcription, quantitative PCR has become a
powerful tool for characterizing gene expression. Multiplex qPCR facilitates
simultaneous amplification of multiple targets by incorporating more than
one primer pair in a single reaction. This reduces the number of reactions
required for the measurement of multiple transcripts and improves the
quality of the experimental data because all the transcripts are measured in
the same well. The StemElite' Gene Expression System is a novel real-time
PCR system for the detection and relative quantitation of RNA expression
levels. The system utilizes validated primer sets to monitor stem cell
differentiation using multiplexed amplification.
 This webinar
was presented by Carl A. Strayer, Ph.D., Technical Services Scientist,
Promega Corporation, USA
This webinar was
presented in the past so registration is not possible
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