The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
The title of this webinar is "Protein expression in
E.coli: Practical steps to pure protein".
is a Basic webinar,
these webinars address the basics of
specific topics and technologies, such as genetic
reporters or apoptosis. They are especially useful for
students, technicians and junior scientists in general.
The focus of biological research
is now moving toward a systematic characterization of genes and their
encoded proteins from the perspective of their expression and function in
cells (proteomics). Proteomics encompasses a wide variety of experiments to
determine the function and interactions of a protein of interest. Many of
these experiments require large quantities of pure protein.
Recombinant protein expression in E. coli can be a powerful method for
producing large quantities of protein quickly, but it also can be an
exercise in frustration. Eukaryotic proteins often show low solubility, low
yield, or incorrect folding in E.coli. Choice of an appropriate affinity
tag, E.coli strain and growth conditions have profound implications for the
success of your protein expression experiment.
This webinar will provide an introduction to E.coli protein expression,
examine affinity tags and E.coli strains, and give some direction in
criteria for choosing the best system for an experiment. We will review
various applications for which affinity tags are often used and the
associated protein purification methods. Optimization of culture growth and
protein purification will be covered in detail.
was presented by Lynn Litterer Ph.D, Technical Services, Promega
This webinar was presented in the past so registration is not possible