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The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "Functional Protein
Analysis in DNA-Based Coupled
Transcription/Translation Cell-free Systems".
This is a
Specialist webinar,
these webinars addresses more specialized
topics and are useful for PhD students and senior
scientists.
Information
Cell-free protein
expression systems, also known as in vitro translation systems, are
alternatives to cell-based protein expression methods. They provide a quick
access to recombinant proteins with advantages include (1) no requirement
for cell culture making them faster and easier to use (2) the ability to
express proteins that are difficult or toxic to express in cell culture (3)
an open format that allows for the addition of auxiliary components such as
labels that can facilitate downstream detection or applications such as
structural analyses and (4) the possibility of parallel synthesis of many
proteins for screening. The template of cell-free protein synthesis can be
DNA or RNA, where DNA based systems provide a much simplified approach by
eliminating the in vitro RNA synthesis step. In this presentation, we will
describe four DNA based cell-free protein expression systems prepared from
different organisms (S30 extract from E. coli, insect cell extract, wheat
germ extract and rabbit reticulocyte lysate) for protein expression and
functional analysis.
We will demonstrate functional protein kinase expression and studies of
protein-protein interactions in these systems in combination with HaloTag®
fusion protein technology.
 This webinar
was presented by Kate Qin Zhao, Ph.D., Sr. Research
Scientist, R&D Protein Analysis, Promega
Corporation
This webinar was
presented in the past so registration is not possible
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