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The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "HaloTag® Protein
Arrays".
This is a
Specialist webinar,
these webinars addresses more specialized
topics and are useful for PhD students and senior
scientists.
HaloTag Technology: a
multifunctional technology enabling protein purification, analysis of
protein interactions, cellular imaging, and surface display for overall
study of protein function
In the post-genomic era a major objective is
to fully characterize the human proteome involving identification of
proteins, determination of their localization, modifications, interactions,
activities and ultimately their function. Essential to this ambition is
development of new affinity and imaging tools allowing comprehensive
analysis of protein function. Here we present a new technology based upon
the use of a protein fusion tag, termed HaloTag, which allows for highly
specific, oriented, and covalent immobilization on surfaces, and also
protein labeling based on covalent binding with a variety of fluorescent
ligands. Because of the multifunctional nature of HaloTag technology,
analysis of many different aspects of protein function can be successfully
achieved using a single construct.
Abstract: "HaloTag® Protein Arrays"
Protein microarray technologies
are increasingly being employed to study protein-protein interactions,
enzyme activities, protein profiling, and antibody screening. Despite great
promises, applications of protein arrays in functional proteomics have
lagged because of time consuming steps required for generating purified
protein content, difficulties in capturing proteins at high density in
stable conformation and need for complex robotics for arraying proteins. To
address the needs of scientists interested in medium throughput custom
protein arrays we used HaloTag multifunctional technology to provide a
HaloLink' Array platform for covalent and oriented capture of proteins on
solid substrates directly from the protein expression systems without any
prior purification step. HaloLink' Arrays use glass slides coated with
polyethylene glycol polymer known to resist non-specific adsorption of
unwanted protein and prevent surface-induced denaturation of specific
proteins. An easily removable silicone gasket is used to create 50 wells on
the glass slides, allowing multiple assays to be performed manually on same
slide without any specialized equipment.
To create a custom protein array, proteins of interest are expressed as
HaloTag fusion (in cell-free or cell based protein expression systems) and
then covalently captured on a hydrogel coated HaloLink' glass slide by
simple 1hr incubation. Silicone gasket allows upto 50 different proteins to
be captured in parallel for multiplexed studies. HaloLink' arrays have been
used for protein:protein and protein:DNA interaction studies as well as
capture of protein biomarkers related to autoimmune diseases. The ability to
easily create a protein array will allow proteomics researchers to shift
their efforts from creating arrays to studying interesting biological
questions using arrays.
HaloTag Webinar Series
This webinar is one of the webinars of the HaloTag series. Please find below
a list of all the webinars of this series, click on the title for
information and registration:
 This webinar
was presented by Nidhi Nath, Ph.D., Sr. Research Scientist R&D, Promega
Corporation
This webinar was
presented in the past so registration is not possible
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