|
|
The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "HaloTag Technology for
Live Cell Imaging". This is a
specialist webinar.
This is a
Specialist webinar,
these webinars addresses more specialized
topics and are useful for PhD students and senior
scientists.
HaloTag Technology: a
multifunctional technology enabling protein purification, analysis of
protein interactions, cellular imaging, and surface display for overall
study of protein function
In the post-genomic era a major objective is
to fully characterize the human proteome involving identification of
proteins, determination of their localization, modifications, interactions,
activities and ultimately their function. Essential to this ambition is
development of new affinity and imaging tools allowing comprehensive
analysis of protein function. Here we present a new technology based upon
the use of a protein fusion tag, termed HaloTag, which allows for highly
specific, oriented, and covalent immobilization on surfaces, and also
protein labeling based on covalent binding with a variety of fluorescent
ligands. Because of the multifunctional nature of HaloTag technology,
analysis of many different aspects of protein function can be successfully
achieved using a single construct.
Abstract: "HaloTag Technology
for Live Cell Imaging"
This presentation will give an overview of HaloTag® technology for imaging,
highlighting its inherent strengths as an imaging tool. Using this
technology any protein of interest, expressed as a HaloTag® fusion can be
specifically and efficiently labeled with a choice of spectrally distinct
fluorescent tags. These small tags, called ligands, are comprised of two
parts; a linker that covalently binds to the HaloTag® protein and a
fluorescent moiety. Given that the ligands for imaging are of distinct
wavelengths and include cell permeant and non-permeant options, this system
affords great flexibility. Not only does it allow "color" choice for each
experiment, but also the option of using two distinct ligands in pulse-chase
fashion, allowing direct observation of protein turn-over or trafficking
within live cells. In addition, the covalent bond that forms between HaloTag®
protein and the ligand withstands denaturation such that reliable fixed cell
and gel-based analyses are possible. Lastly, the chemistry involved in
creating a ligand is quite simple and accommodates the creation of targeted
dyes of any kind. This will be shown in the development and successful
cellular targeting of a micro environmental sensor and a dye attuned to high
resolution microscopy.
HaloTag Webinar Series
This webinar is one of the webinars of the HaloTag series. Please find below
a list of all the webinars of this series, click on the title for
information and registration:
 This webinar
was presented by Hélène Beninck, Ph.D., Sr. Research Scientist R&D,
Promega Corporation
This webinar was
presented in the past so registration is not possible
|
|