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Promega @cademy webinar:

Robust Purification of Arginine Methyltransferase (CARM1) from Mammalian Cells Using HaloTag Technology 


The Promega @cademy provides a tailored programme of non-commercial live webinars, covering a wide range of scientific topics, from very basic concepts to highly technical research presentations. We would like to invite you to download the recording for the free webinar, which was held on Monday July 18, 2011. The title is: "Robust Purification of Arginine Methyltransferase (CARM1) from Mammalian Cells Using HaloTag Technology".


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Identification and characterization of post-translational modifications and protein function largely depends on success in purification of full-length target protein. CARM1 is a mammalian transcription factor that plays a role in progression of breast cancer. CARM1 is able to methylate itself; however, this process has been previously uncharacterized, due to difficulty in purifying the full-length protein. HaloTag technology enabled the purification of full-length, active CARM1 from mammalian cells; the purified protein was used for both mass spectrometry and functional analysis to characterize the site of automethylation.  This work represents the first identified physiological role for automethylation in the protein arginine methyltransferase family.


This webinar is be presented by Robert S. Chumanov PhD.

Robert S. Chumanov received his PhD in Cellular and Molecular Biology in 2009 from University of Wisconsin -Madison (United States) where he specialized in protein purification in the laboratory of Dr. Richard Burgess. His highly collaboration-driven research was based on using protein purification to address critical questions in diverse areas of molecular biology ranging from virology to neurobiology to oncology. He has extensive experience adopting various prokaryotic, insect, and mammalian systems for protein expression and purification. More recently he adopted the HaloTag technology to tackle production of methyltransferase enzymes from mammalian cells for functional and structural characterization.




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