The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
We would like to invite you to download the
recording for the free
webinar, which was held on Monday July
18, 2011. The title is: "Robust
Purification of Arginine Methyltransferase
(CARM1) from Mammalian Cells Using HaloTag
This is a
specialist webinar. Specialist webinars address
more specialized topics and are useful for PhD
students and senior scientists.
Identification and characterization of post-translational modifications and
protein function largely depends on success in purification of full-length
target protein. CARM1 is a mammalian transcription factor that plays a role
in progression of breast cancer. CARM1 is able to methylate itself; however,
this process has been previously uncharacterized, due to difficulty in
purifying the full-length protein. HaloTag technology enabled the
purification of full-length, active CARM1 from mammalian cells; the purified
protein was used for both mass spectrometry and functional analysis to
characterize the site of automethylation.
work represents the first identified physiological role for automethylation
in the protein arginine methyltransferase family.
This webinar is be presented by Robert S. Chumanov PhD.
S. Chumanov received his PhD in Cellular and Molecular Biology in 2009 from
University of Wisconsin -Madison (United States) where he specialized in
protein purification in the laboratory of Dr. Richard Burgess. His highly
collaboration-driven research was based on using protein purification to
address critical questions in diverse areas of molecular biology ranging
from virology to neurobiology to oncology. He has extensive experience
adopting various prokaryotic, insect, and mammalian systems for protein
expression and purification. More recently he adopted the HaloTag technology
to tackle production of methyltransferase enzymes from mammalian cells for
functional and structural characterization.