The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
The title of this webinar is "Analysis and
molecular detection of microsatellite
instability in MSI-H type cancer cells"
This is a
these webinars addresses more specialized
topics and are useful for PhD students and senior
of microsatellites instability is abnormal variation of repetitive
nucleotide sequences in DNA from the tumor compared to DNA of healthy tissue
from the same individual.
whether a tumor exhibits microsatellite instability (MSI) is useful in
identifying patients with hereditary non-polyposis colorectal cancer (HNPCC)
and sporadic gastrointestinal cancers with defective mismatch repair system
(MMR). There is evidence to suggest that colorectal cancer with MSI-H
respond differently to adjuvant chemotherapy so the assessment of MSI-H
status aids in establishing a clinical prognosis.
developed, based on previous studies done by Dr Hamelin and his group, the
MSI analysis system. This fluorescent multiplex PCR system meets the 2002
NCI workshop recommendations and includes five mononucleotides markers
(BAT-25, BAT-26, NR-21, NR-24 and MONO-27). In addition two pentanucleotide
markers were added to identify sample mixs-up and/or contamination. PCR
products are separated by capillary electrophoresis. A MSI analysis macro
software simplifies and standardizes allele size determination for
consistent data analysis.
procedure from DNA extraction to MSI analysis will be described during the
This webinar was presented by Isabelle Baginski Ph.D., Technical Services Manager, Promega
This webinar was
presented in the past so registration is not possible