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The Promega @cademy provides a tailored
programme of non-commercial live webinars,
covering a wide range of scientific topics, from
very basic concepts to highly technical research
presentations.
The title of this webinar is: "Why
should I use Bioluminescent Reporter Genes"
 This
is a Basic webinar,
these webinars address the basics of
specific topics and technologies, such as genetic
reporters or apoptosis. They are especially useful for
students, technicians and junior scientists in general.
Information
Genetic reporters are very valuable tools to research. The use of
bioluminescence (emission of light that follows the absorption of energy
generated by an enzymatically-catalyzed biochemical reaction) as a read-out
for genetic reporters (luciferase reporter genes) raises great advantages in
terms of sensitivity, background, dynamic range and experimental conditions.
The aim of this webinar is first to explain the principle of luciferase
reporter assays and how they can be used to generate more reliable data
(normalization), and then to review different applications of the luciferase
genetic reporter technology using experimental examples. Finally, this
webinar addresses step-by-step the important considerations to set up a
luciferase genetic reporter assay.
This webinar was
presented in the past so registration is not possible
Additional Tools
Introduction to
Bioluminescence Assays
This animation shows how we exploit the chemistry of the bioluminescent
reaction to create a suite of robust and sensitive biological assays.
Genetic Reporter Assays, ATP Assays, and Luciferin Substrate Assays are
presented(view
animation).
Introduction to Reporter Gene Assays
This animation introduces the concept of reporter gene assays, using the
Dual-Luciferase® Assay as a model (view
animation). |